Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13313(B)] to PHYHD1
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
Product nameAnti-PHYHD1 antibody [EPR13313(B)]
See all PHYHD1 primary antibodies
DescriptionRabbit monoclonal [EPR13313(B)] to PHYHD1
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PHYHD1 aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: Q5SRE7
- Human fetal liver andHuman fetal kidney lysates. HepG2 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab184174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 32 kDa.|
|ICC/IF||1/250 - 1/500.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionProbably acts as alpha-ketoglutatare-dependent dioxygenase.
Sequence similaritiesBelongs to the PhyH family. PHYHD1 subfamily.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- MGC16638 antibody
- OTTHUMP00000022302 antibody
- OTTHUMP00000022303 antibody
All lanes : Anti-PHYHD1 antibody [EPR13313(B)] (ab184174) at 1/10000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 32 kDa
5% NFDM/TBST blocking/diluting buffer
Flow ctometric analysis of HepG2 cells fixed in 2% paraformaldehyde labeling PHYHD1 with ab184174 at 1/400 dilution (red). Goat andti rabbit IgG (FITC) was used at 1/150 dilution as secondary antibody. Rabbit monoclonal IgG was used as an isotype control (green).
Immunofluorescent analysis of HepG2 cells fixed in 4% paraformaldehyde labeling PHYHD1 with ab184174 at 1/500 dilution. Goat anti-rabbit IgG(Alexa Fluor® 555) was used at 1/200 dilution for secondary antibody and the cells were counterstained with DAPI.
ab184174 has not yet been referenced specifically in any publications.