Key features and details
- Goat polyclonal to PI-15
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PI-15 antibody
DescriptionGoat polyclonal to PI-15
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Pig, Zebrafish
- HeLa cell lysate
Previously labelled as Protease Inhibitor 15.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab133172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 29 kDa).
1 hour primary incubation is recommended for this product.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionSerine protease inhibitor which displays weak inhibitory activity against trypsin.
Tissue specificityWeakly expressed. Expressed at low level in prostate, mammary gland, salivary gland and thyroid gland.
Sequence similaritiesBelongs to the CRISP family.
- Information by UniProt
- 25 kDa trypsin inhibitor antibody
- CRISP-8 antibody
- CRISP8 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staing PI-15 in Human Skeletal Muscle with ab133172 at 5 µg/ml
Anti-PI-15 antibody (ab133172) at 0.3 µg/ml + HeLa cell lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 29 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
ab133172 has not yet been referenced specifically in any publications.