Recombinant Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (ab183957)
![Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (ab183957)](https://www.abcam.com/ps/products/183/ab183957/Images/ab183957-285157-GDBKOWB.jpg "Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (ab183957)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19693] to PI 3 Kinase catalytic subunit alpha/PIK3CA
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693]
See all PI 3 Kinase catalytic subunit alpha/PIK3CA primary antibodies -
Description
Rabbit monoclonal [EPR19693] to PI 3 Kinase catalytic subunit alpha/PIK3CA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: F9 and M1 whole cell lysates; Mouse hypothalamus and fetal brain lysates; P0 Rat brain lysate. IP: Mouse hypothalamus lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19693 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 124 kDa).
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| IP |
1/30.
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 124 kDa). |
|
IP
1/30. |
Target
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Function
Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. -
Involvement in disease
Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500].
Defects in PIK3CA are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
Defects in PIK3CA are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
Defects in PIK3CA may underlie hepatocellular carcinoma (HCC) [MIM:114550].
Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB) [MIM:182000]. A common benign skin tumor. Seborrheic keratoses usually begin with the appearance of one or more sharply defined, light brown, flat macules. The lesions may be sparse or numerous. As they initially grow, they develop a velvety to finely verrucous surface, followed by an uneven warty surface with multiple plugged follicles and a dull or lackluster appearance. -
Sequence similarities
Belongs to the PI3/PI4-kinase family.
Contains 1 C2 domain.
Contains 1 PI3K/PI4K domain. - Information by UniProt
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Database links
- Entrez Gene: 5290 Human
- Entrez Gene: 18706 Mouse
- Entrez Gene: 170911 Rat
- Omim: 171834 Human
- SwissProt: P42336 Human
- SwissProt: P42337 Mouse
- Unigene: 553498 Human
- Unigene: 85701 Human
see all -
Alternative names
- 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha antibody
- 5-bisphosphate 3-kinase catalytic subunit alpha isoform antibody
- caPI3K antibody
see all
Images
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Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (ab183957)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: PI 3 Kinase catalytic subunit alpha/PIK3CA knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Jurkat whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab183957 observed at 125 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab183957 was shown to recognize PI 3 Kinase catalytic subunit alpha/PIK3CA in wild type cells as signal was lost at the expected MW in PI 3 Kinase catalytic subunit alpha/PIK3CA knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PI 3 Kinase catalytic subunit alpha/PIK3CA knockout samples were subjected to SDS-PAGE. ab183957 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunoprecipitation - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (ab183957)](https://www.abcam.com/ps/products/183/ab183957/Images/ab183957-273355-183957IP.jpg)
Immunoprecipitation - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (ab183957)PI 3 Kinase catalytic subunit alpha/PIK3CA was immunoprecipitated from 0.35 mg of mouse hypothalamus lysate with ab183957 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab183957 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse hypothalamus lysate, 10µg (Input).
Lane 2: ab183957 IP in mouse hypothalamus lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab183957 in mouse hypothalamus lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes. -
![Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (ab183957)](https://www.abcam.com/ps/products/183/ab183957/Images/ab183957-273357-183957WBa.jpg)
Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (ab183957)All lanes : Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (ab183957) at 1/1000 dilution
Lane 1 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate
Lane 2 : M1 (Mouse myeloblast myeloid leukemia cell line) whole cell lysate
Lane 3 : Mouse hypothalamus lysate
Lane 4 : Mouse fetal brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 124 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 3 minutes; Lane 2 and 3: 5 seconds; Lane 4: 3 seconds.
The observed molecular weight is consistent with what has been described in the literature (PMID: 19701705).
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![Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (ab183957)](https://www.abcam.com/ps/products/183/ab183957/Images/ab183957-273356-183957WBb.jpg)
Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (ab183957)Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (ab183957) at 1/1000 dilution + P0 Rat brain lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 124 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
The observed molecular weight is consistent with what has been described in the literature (PMID: 19701705).
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![Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (ab183957)](https://www.abcam.com/ps/products/183/ab183957/Images/ab183957-5-benefits-of-recombinant-antibodies.png)
Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (ab183957)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab183957 has been referenced in 1 publication.
- Li G et al. Acupuncture Administration Improves Cognitive Functions and Alleviates Inflammation and Nuclear Damage by Regulating Phosphatidylinositol 3 Kinase (PI3K)/Phosphoinositol-Dependent Kinase 1 (PDK1)/Novel Protein Kinase C (nPKC)/Rac 1 Signaling Pathway in Senescence-Accelerated Prone 8 (SAM-P8) Mice. Med Sci Monit 25:4082-4093 (2019). PubMed: 31152645
