Recombinant
RabMAb

Recombinant Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] - BSA and Azide free (ab243914)

Overview

  • Product name

    Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] - BSA and Azide free
    See all PI 3 Kinase catalytic subunit alpha/PIK3CA primary antibodies
  • Description

    Rabbit monoclonal [SP139] to PI 3 Kinase catalytic subunit alpha/PIK3CA - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Chicken, Cow
  • Immunogen

    Synthetic peptide within Human PI 3 Kinase catalytic subunit alpha/PIK3CA aa 500-600 (internal sequence). The exact sequence is proprietary.
    Database link: P42336

  • Positive control

    • IHC: Human breast carcinoma tissue FC: HeLa, and C6 cells. ICC/IF: HeLa, and C6 cells.
  • General notes

    Ab243914 is the carrier-free version of ab135384. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab243914 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab243914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Perform heat mediated antigen retrieval by boiling tissue section in 1mM EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. Incubate with primary antibody for 10 minutes at room temperature.

Target

  • Function

    Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
  • Involvement in disease

    Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500].
    Defects in PIK3CA are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
    Defects in PIK3CA are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
    Defects in PIK3CA may underlie hepatocellular carcinoma (HCC) [MIM:114550].
    Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB) [MIM:182000]. A common benign skin tumor. Seborrheic keratoses usually begin with the appearance of one or more sharply defined, light brown, flat macules. The lesions may be sparse or numerous. As they initially grow, they develop a velvety to finely verrucous surface, followed by an uneven warty surface with multiple plugged follicles and a dull or lackluster appearance.
  • Sequence similarities

    Belongs to the PI3/PI4-kinase family.
    Contains 1 C2 domain.
    Contains 1 PI3K/PI4K domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha antibody
    • 5-bisphosphate 3-kinase catalytic subunit alpha isoform antibody
    • caPI3K antibody
    • CLOVE antibody
    • CWS5 antibody
    • MCAP antibody
    • MCM antibody
    • MCMTC antibody
    • MGC142161 antibody
    • MGC142163 antibody
    • p110 alpha antibody
    • p110alpha antibody
    • Phosphatidylinositol 3 kinase catalytic alpha polypeptide antibody
    • Phosphatidylinositol 3 kinase catalytic 110 KD alpha antibody
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha antibody
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha isoform antibody
    • Phosphatidylinositol 4,5 bisphosphate 3 kinase 110 kDa catalytic subunit alpha antibody
    • Phosphatidylinositol-4 antibody
    • Phosphoinositide 3 kinase catalytic alpha polypeptide antibody
    • PI 3 Kinase catalytic subunit alpha antibody
    • PI3 kinase p110 subunit alpha antibody
    • PI3-kinase subunit alpha antibody
    • PI3K antibody
    • PI3K-alpha antibody
    • PI3KC A antibody
    • PIK3C A antibody
    • Pik3ca antibody
    • PK3CA antibody
    • PK3CA_HUMAN antibody
    • PtdIns 3 kinase p110 antibody
    • PtdIns-3-kinase subunit alpha antibody
    • PtdIns-3-kinase subunit p110-alpha antibody
    • Serine/threonine protein kinase PIK3CA antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence analysis of C6 (rat glial tumor glial cell) cells labeling PI 3 Kinase catalytic subunit alpha/PIK3CA with purified ab135384 at 1:25(10 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225716).
  • Flow Cytometry analysis of C6(Rat glial tumor glial cell) cells labeling PI 3 Kinase catalytic subunit alpha/PIK3CA with purified ab135384 at 1:250 dilution (1.00 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225716).
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labelling PI 3 Kinase catalytic subunit alpha/PIK3CA with ab135384 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab135384).

  • Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PI 3 Kinase catalytic subunit alpha/PIK3CA with purified ab135384 at 1:25(10 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab135384).
  • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PI 3 Kinase catalytic subunit alpha/PIK3CA with purified ab135384 at 1:250 dilution (1.00 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab135384).

References

ab243914 has not yet been referenced specifically in any publications.

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