Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab154598)
Key features and details
- Rabbit polyclonal to PI 3 Kinase catalytic subunit gamma/PI3K-gamma
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody
See all PI 3 Kinase catalytic subunit gamma/PI3K-gamma primary antibodies -
Description
Rabbit polyclonal to PI 3 Kinase catalytic subunit gamma/PI3K-gamma -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human PI 3 Kinase catalytic subunit gamma/PI3K-gamma aa 1041-1102 (C terminal).
Database link: P48736 -
Positive control
- WB: Transfected HEK-293T, Neuro 2A, RAW264.7, C8D30 and PC-12 cell lysate; IHC-P: Human breast cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 59.99% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 126 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 126 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
Target
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Function
3-phosphorylates the cellular phosphoinositide PtdIns-4,5-biphosphate (PtdIns(4,5)P2) to produce PtdIns-3, 4,5-triiphosphate (PtdIns(3,4,5)P3). Links G-protein coupled receptor activation to the secondary messenger PtdIns(3,4,5)P3 production. -
Tissue specificity
Pancreas, skeletal muscle, liver and heart. -
Pathway
Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis. -
Sequence similarities
Belongs to the PI3/PI4-kinase family.
Contains 1 PI3K/PI4K domain. - Information by UniProt
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Database links
- Entrez Gene: 5294 Human
- Entrez Gene: 30955 Mouse
- Entrez Gene: 298947 Rat
- GenBank: AF327656 Human
- Omim: 601232 Human
- SwissProt: P48736 Human
- SwissProt: Q9JHG7 Mouse
- Unigene: 32942 Human
see all -
Alternative names
- 1 phosphatidylinositol 3 kinase antibody
- 5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma antibody
- 5-bisphosphate 3-kinase catalytic subunit gamma isoform antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue staining PI 3 Kinase catalytic subunit gamma with ab154598 at 1/100 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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All lanes : Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab154598) at 1/500 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : PI3K-gamma-transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 126 kDa
7.5% SDS PAGE -
Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab154598) at 1/1000 dilution + PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg
Predicted band size: 126 kDa7.5% SDS-PAGE
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All lanes : Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab154598) at 1/500 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : C8-D30 (mouse astrocyte type III cerebellum cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 126 kDa5% SDS-PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (24)
ab154598 has been referenced in 24 publications.
- Huang S et al. LINC02381 aggravates breast cancer through the miR-1271-5p/FN1 axis to activate PI3K/AKT pathway. Mol Carcinog 61:346-358 (2022). PubMed: 34882856
- Jia M et al. Inhibition of PI3K/AKT/mTOR Signalling Pathway Activates Autophagy and Suppresses Peritoneal Fibrosis in the Process of Peritoneal Dialysis. Front Physiol 13:778479 (2022). PubMed: 35309056
- Zhan CH et al. The cancer-testis antigen a-kinase anchor protein 3 facilitates breast cancer progression via activation of the PTEN/PI3K/AKT/mTOR signaling. Bioengineered 13:8478-8489 (2022). PubMed: 35322748
- Wu T et al. Effects of Qidantang Granule on early stage of diabetic kidney disease in rats. Aging (Albany NY) 14:4888-4896 (2022). PubMed: 35696643
- Chen X et al. MiR-21 regulating PVT1/PTEN/IL-17 axis towards the treatment of infectious diabetic wound healing by modified GO-derived biomaterial in mouse models. J Nanobiotechnology 20:309 (2022). PubMed: 35764963