Overview

  • Product name

    Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody
    See all PI 3 Kinase catalytic subunit gamma/PI3K-gamma primary antibodies
  • Description

    Rabbit polyclonal to PI 3 Kinase catalytic subunit gamma/PI3K-gamma
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human PI 3 Kinase catalytic subunit gamma/PI3K-gamma (C terminal) conjugated to keyhole limpet haemocyanin.

  • Positive control

    • HeLa cell lysate; Paraffin embedded Human muscle tissue
  • General notes

    Previously labelled as PI 3 Kinase catalytic subunit gamma. 

Properties

Applications

Our Abpromise guarantee covers the use of ab70912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Detects a band of approximately 126 kDa.
ELISA 1/1000.
IHC-P 1/10 - 1/50.

Target

  • Function

    3-phosphorylates the cellular phosphoinositide PtdIns-4,5-biphosphate (PtdIns(4,5)P2) to produce PtdIns-3, 4,5-triiphosphate (PtdIns(3,4,5)P3). Links G-protein coupled receptor activation to the secondary messenger PtdIns(3,4,5)P3 production.
  • Tissue specificity

    Pancreas, skeletal muscle, liver and heart.
  • Pathway

    Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
  • Sequence similarities

    Belongs to the PI3/PI4-kinase family.
    Contains 1 PI3K/PI4K domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1 phosphatidylinositol 3 kinase antibody
    • 5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma antibody
    • 5-bisphosphate 3-kinase catalytic subunit gamma isoform antibody
    • p110 gamma antibody
    • p110gamma antibody
    • p120 PI3K antibody
    • p120-PI3K antibody
    • Phosphatidylinositol 3 kinase catalytic 110 kD gamma antibody
    • Phosphatidylinositol 3 kinase gamma, p110 gamma antibody
    • Phosphatidylinositol 3 kinase, catalytic, gamma polypeptide antibody
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit gamma antibody
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit gamma antibody
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit gamma isoform antibody
    • Phosphatidylinositol-4 antibody
    • Phosphoinositide 3 kinase catalytic gamma polypeptide antibody
    • Phosphoinositide 3 kinase gamma catalytic subunit antibody
    • PI 3 Kinase catalytic subunit gamma antibody
    • PI3 kinase p110 subunit gamma antibody
    • PI3-kinase subunit gamma antibody
    • PI3CG antibody
    • PI3K antibody
    • PI3K-gamma antibody
    • PI3Kgamma antibody
    • PIK3 antibody
    • Pik3cg antibody
    • PK3CG_HUMAN antibody
    • PtdIns-3-kinase subunit gamma antibody
    • PtdIns-3-kinase subunit p110-gamma antibody
    • Serine/threonine protein kinase PIK3CG antibody
    see all

Images

  • Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab70912) at 1/60 dilution + HeLa cell line lysate at 35 µg

    Observed band size: 126 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab70912) at 1/60 dilution

    Lane 1 : Nontransfected 293 cell line lysate
    Lane 2 : 293 cell line lysate transiently transfected with the PI 3 Kinase catalytic subunit gamma/PI3K-gamma gene

    Lysates/proteins at 2 µg per lane.

    Observed band size: 126 kDa why is the actual band size different from the predicted?
    Additional bands at: 36 kDa, 40 kDa, 50 kDa. We are unsure as to the identity of these extra bands.

  • ab70912, at a 1/10 dilution, staining human PI 3 Kinase catalytic subunit gamma/PI3K-gamma in muscle, using Immunohistochemistry, Formalin/PFA fixed Paraffin embedded tissue. ab70912 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

References

This product has been referenced in:

  • Lu X & Qian J Downregulated MEG3 participates in rheumatoid arthritis via promoting proliferation of fibroblast-like synoviocytes. Exp Ther Med 17:1637-1642 (2019). Read more (PubMed: 30783431) »
  • Conte E  et al. Inhibition of PI3K Prevents the Proliferation and Differentiation of Human Lung Fibroblasts into Myofibroblasts: The Role of Class I P110 Isoforms. PLoS One 6:e24663 (2011). WB ; Human . Read more (PubMed: 21984893) »
See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab70912.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up