Overview

  • Product name

    Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [Y388]
    See all PI 3 Kinase catalytic subunit gamma/PI3K-gamma primary antibodies
  • Description

    Rabbit monoclonal [Y388] to PI 3 Kinase catalytic subunit gamma/PI3K-gamma
  • Host species

    Rabbit
  • Specificity

    This antibody is specific for PI 3 Kinase catalytic subunit gamma/PI3K-gamma.

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC or IHC
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PI 3 Kinase catalytic subunit gamma/PI3K-gamma aa 1-100 (N terminal). The exact sequence is proprietary.

  • Epitope

    ab32089 reacts with an epitope located in the N terminal region of PI 3 Kinase catalytic subunit gamma/PI3K-gamma
  • Positive control

    • WB: HepG2 lysate and Jurkat whole cell lysate (ab7899)..
  • General notes

    Previously labelled as PI 3 Kinase catalytic subunit gamma. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab32089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 126 kDa.
  • Application notes
    Is unsuitable for ICC or IHC.
  • Target

    • Function

      3-phosphorylates the cellular phosphoinositide PtdIns-4,5-biphosphate (PtdIns(4,5)P2) to produce PtdIns-3, 4,5-triiphosphate (PtdIns(3,4,5)P3). Links G-protein coupled receptor activation to the secondary messenger PtdIns(3,4,5)P3 production.
    • Tissue specificity

      Pancreas, skeletal muscle, liver and heart.
    • Pathway

      Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
    • Sequence similarities

      Belongs to the PI3/PI4-kinase family.
      Contains 1 PI3K/PI4K domain.
    • Information by UniProt
    • Database links

    • Alternative names

      • 1 phosphatidylinositol 3 kinase antibody
      • 5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma antibody
      • 5-bisphosphate 3-kinase catalytic subunit gamma isoform antibody
      • p110 gamma antibody
      • p110gamma antibody
      • p120 PI3K antibody
      • p120-PI3K antibody
      • Phosphatidylinositol 3 kinase catalytic 110 kD gamma antibody
      • Phosphatidylinositol 3 kinase gamma, p110 gamma antibody
      • Phosphatidylinositol 3 kinase, catalytic, gamma polypeptide antibody
      • Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit gamma antibody
      • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit gamma antibody
      • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit gamma isoform antibody
      • Phosphatidylinositol-4 antibody
      • Phosphoinositide 3 kinase catalytic gamma polypeptide antibody
      • Phosphoinositide 3 kinase gamma catalytic subunit antibody
      • PI 3 Kinase catalytic subunit gamma antibody
      • PI3 kinase p110 subunit gamma antibody
      • PI3-kinase subunit gamma antibody
      • PI3CG antibody
      • PI3K antibody
      • PI3K-gamma antibody
      • PI3Kgamma antibody
      • PIK3 antibody
      • Pik3cg antibody
      • PK3CG_HUMAN antibody
      • PtdIns-3-kinase subunit gamma antibody
      • PtdIns-3-kinase subunit p110-gamma antibody
      • Serine/threonine protein kinase PIK3CG antibody
      see all

    Images

    • All lanes : Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [Y388] (ab32089) at 1/1000 dilution (Purified)

      Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 15 µg


      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 126 kDa
      Observed band size: 110 kDa
      why is the actual band size different from the predicted?

    • Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [Y388] (ab32089) at 1/1000 dilution + Jurkat cell lysate.

      Predicted band size: 126 kDa
      Observed band size: 110 kDa why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Guan Y  et al. Chloride channel-3 is required for efficient tumour cell migration and invasion in human cervical squamous cell carcinoma. Gynecol Oncol 153:661-669 (2019). Read more (PubMed: 30905432) »
    • Tang KL  et al. PAR-2 promotes cell proliferation, migration, and invasion through activating PI3K/AKT signaling pathway in oral squamous cell carcinoma. Biosci Rep 39:N/A (2019). Read more (PubMed: 31213575) »
    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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