Anti-PI 3 Kinase Class 3 antibody [EPR5301] (ab124905)


  • Product name
    Anti-PI 3 Kinase Class 3 antibody [EPR5301]
    See all PI 3 Kinase Class 3 primary antibodies
  • Description
    Rabbit monoclonal [EPR5301] to PI 3 Kinase Class 3
  • Host species
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PI 3 Kinase Class 3 aa 800-900. The exact sequence is proprietary.

  • Positive control
    • L6, C2C12, 293T, SHYSY-5Y cell lysates; Mouse brain tissue lysate; Human gastric adenocarcinoma tissue
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab124905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 102 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis.
    • Tissue specificity
      Ubiquitously expressed, with a highest expression in skeletal muscle.
    • Sequence similarities
      Belongs to the PI3/PI4-kinase family.
      Contains 1 PI3K/PI4K domain.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • hVps34 antibody
      • MGC61518 antibody
      • Phosphatidylinositol 3 kinase catalytic subunit type 3 antibody
      • Phosphatidylinositol 3 kinase class 3 antibody
      • Phosphatidylinositol 3 kinase p100 subunit antibody
      • Phosphatidylinositol 3-kinase catalytic subunit type 3 antibody
      • Phosphatidylinositol 3-kinase p100 subunit antibody
      • Phosphoinositide 3 kinase class 3 antibody
      • Phosphoinositide-3-kinase class 3 antibody
      • PI3 kinase type 3 antibody
      • PI3-kinase type 3 antibody
      • PI3K type 3 antibody
      • Pik3c3 antibody
      • PK3C3_HUMAN antibody
      • PtdIns 3 kinase type 3 antibody
      • PtdIns-3-kinase type 3 antibody
      • Vps 34 antibody
      • Vps34 antibody
      see all


    • All lanes : Anti-PI 3 Kinase Class 3 antibody [EPR5301] (ab124905) at 1/1000 dilution

      Lane 1 : L6 cell lysate
      Lane 2 : C2C12 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : Mouse brain tissue lysate
      Lane 5 : SHSY-5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 102 kDa
      Observed band size: 100 kDa (why is the actual band size different from the predicted?)

    • ab124905, at 1/50 dilution, staining PI 3 Kinase in paraffin-embedded Human gastric adenocarcinoma tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD


    This product has been referenced in:
    • Kimura H  et al. Vps34 regulates myofibril proteostasis to prevent hypertrophic cardiomyopathy. JCI Insight 2:e89462 (2017). Read more (PubMed: 28097232) »
    • Sun Y  et al. TMEM74 promotes tumor cell survival by inducing autophagy via interactions with ATG16L1 and ATG9A. Cell Death Dis 8:e3031 (2017). Read more (PubMed: 29048433) »

    See all 5 Publications for this product

    Customer reviews and Q&As

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