Overview

  • Product name
    Anti-PI 3 Kinase p110 delta antibody
    See all PI 3 Kinase p110 delta primary antibodies
  • Description
    Rabbit polyclonal to PI 3 Kinase p110 delta
  • Host species
    Rabbit
  • Specificity
    Binds to a 110 Kd protein. Not likely to cross react with other isoforms due to sequence dissimilarity.
  • Tested applications
    Suitable for: ICC/IF, IP, WB, ELISA, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    20 amino acid C-terminal peptide to Phosphoinositide 3 Kinase p110 delta

  • Positive control
    • ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab1678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IP Use at an assay dependent concentration. PubMed: 23023391
WB 1/2000 - 1/3000.
ELISA 1/75000.
ICC 1/400.

Target

  • Tissue specificity
    Expressed predominantly in leukocytes.
  • Pathway
    Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
  • Sequence similarities
    Belongs to the PI3/PI4-kinase family.
    Contains 1 PI3K/PI4K domain.
  • Post-translational
    modifications
    Autophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5-bisphosphate 3-kinase 110 kDa catalytic subunit delta antibody
    • 5-bisphosphate 3-kinase catalytic subunit delta isoform antibody
    • APDS antibody
    • GRB1 antibody
    • IMD14 antibody
    • p110d antibody
    • p110delta antibody
    • p110dp85a antibody
    • p85-ALPHA antibody
    • Phosphatidylinositol 3 kinase catalytic delta polypeptide antibody
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit delta isoform antibody
    • Phosphatidylinositol 4,5 bisphosphate 3 kinase 110 kDa catalytic subunit delta antibody
    • Phosphatidylinositol 4,5 bisphosphate 3 kinase, catalytic subunit delta antibody
    • Phosphatidylinositol 45 bisphosphate 3 kinase catalytic subunit delta isoform antibody
    • Phosphatidylinositol-4 antibody
    • Phosphoinositide 3 kinase B antibody
    • Phosphoinositide 3 kinase C antibody
    • Phosphoinositide 3 kinase catalytic delta polypeptide antibody
    • Phosphoinositide 3 kinase, catalytic, delta polypeptide variant p37delta antibody
    • PI3 kinase p110 subunit delta antibody
    • PI3-kinase subunit delta antibody
    • PI3K antibody
    • PI3K-delta antibody
    • PI3Kdelta antibody
    • Pik3cd antibody
    • PIK3R1 antibody
    • PK3CD antibody
    • PK3CD_HUMAN antibody
    • PtdIns 3 kinase p110 antibody
    • PtdIns 3 kinase subunit p110 delta antibody
    • PtdIns-3-kinase subunit delta antibody
    • PtdIns-3-kinase subunit p110-delta antibody
    see all

Images

  • All lanes : Anti-PI 3 Kinase p110 delta antibody (ab1678) at 1/2000 dilution

    Lane 1 : Peripheral Blood Mononuclear Cells (PBMCs)
    Lane 2 : Primary human monocytes
    Lane 3 : Primary human macropahges
    Lane 4 : U937 monocytic cell line
    Lane 5 : Whole human Lung

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Bovine anti-Rabbit IgG-HRP at 1/2000 dilution

    Developed using the ECL technique.

    Exposure time: 1 minute

    See Abreview

  • ICC/IF image of ab1678 stained HeLa (human epithelial cell line from cervix adenocarcinoma) cells. The cells were 4% formaldehyde fixed (10 minutes) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1678, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.

References

This product has been referenced in:
  • Goulielmaki E  et al. Pharmacological inactivation of the PI3K p110d prevents breast tumour progression by targeting cancer cells and macrophages. Cell Death Dis 9:678 (2018). Read more (PubMed: 29880805) »
  • Li J  et al. Down-regulation of microRNA-27b promotes retinal pigment epithelial cell proliferation and migration by targeting Nox2. Pathol Res Pract 214:925-933 (2018). WB ; Human . Read more (PubMed: 29858119) »
See all 12 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (K652 cells)
Loading amount
5000 cells
Specification
K652 cells
Treatment
DMSO or 1 uM CAL-101 for 24 hours
Gel Running Conditions
Reduced Denaturing (4-20% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Mar 21 2013

Question

Hello,

We have a quality complaint for item ab55593. Please check attached file for detail.

We request money back.

Thanks,


Item number: ab55593

Order number: 1049169



General Information

Antibody storage conditions?





-20









Description of the problem (high background, wrong band size, more bands, no band etc.)





no band









Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)





Human/Cell extract









Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)





RIPA buffer containing protease and phosphatase inhibitor cocktails (Roche Diagnostics) and also heating sample







Amount of protein loaded





60, 80μg of total protein extracts









Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)





10% SDS-PAGE gels









Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)





nitrocellulose membranes (Milliopore) 90 min

The membranes were blocked with 5% milk powder, washed with TBST







Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)





Primeary antibody purchased from Abcom named ab55593

Primary antibody is diluted with 4% BSA. And the membranes were incubated overnight at 4℃. The membranes were washed with TBST three times.







Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)





peroxidase-conjugated anti-mouse IgG (Sigma) as a secondary antibody

Incubation for 2 hours was at room temperature.

Also washed three times by TBST.







Detection method (ECL, ECLPlus etc.)





Western Blotting luminal reagent (Santa cruz)







Positive and negative controls used (please specify)





Gapdh as positive control









Instruction

Repeat times: 3


Our western blotting experiment is based on the instruction provided by your company, but we got no band. At the same time, we used the protein of 293T cell (published by some paper) to repeat the experiment and we got the same result.

We have prolonged the time of the incubation and exposure, and also use the Concentration 1:200, but there is still no band of PI3KCB. There is no problem with our technology of western blotting, because we have got the target band of PI3KC3 by antibody from CST.

Finally, I hope that your company can make up our loss and solve the problems as soon as possible.

Read More
Answer

Thank you for your enquiry regarding ab55593 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guarantee period. This means that in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

As the datasheet indicates this antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

Would you be so kind to confirm the sample types used (species, cells, tissues)?

We have several alternative antibodies against the same target (PI3 Kinase p110 beta - ab32569, ab97322, ab1678) and I could offer you a free of charge replacement, or a credit note or a full refund as compensation.

Thank you for your understanding and co-operation in this matter. I look forward to hearing from you and hope to solve this problem as soon as possible.

Read More

Answer

Thank you for your data and for bringing this article to our attention.

The immunogen sequence is

*************

Is that included in the p37delta sequence?

I am glad to see the antibody was specific for the p110 delta isoform. I encourage you to submit your results in an Abreview. There are currently two examples of western blot reviews on the online datasheet.

https://www.abcam.com/pi-3-kinase-p110-delta-antibody-ab1678-reviews.html

For information on how to submit a review (for which you will receive credit for a future purchase or gift card), please see the page at this link:

www.abcam.com/abreviews

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (monocyte, macrophage, whole lung)
Loading amount
20 µg
Specification
monocyte, macrophage, whole lung
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Sep 03 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (cancer cells)
Loading amount
15 µg
Specification
cancer cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Jun 07 2007

Question
Answer

We have no evidence that PI3Kinase ab1678 is a blocking antibody

Read More

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