Product nameAnti-PI 3 Kinase p110 delta antibody
See all PI 3 Kinase p110 delta primary antibodies
DescriptionRabbit polyclonal to PI 3 Kinase p110 delta
SpecificityBinds to a 110 Kd protein. Not likely to cross react with other isoforms due to sequence dissimilarity.
Tested applicationsSuitable for: ICC/IF, IP, WB, ELISA, ICCmore details
Species reactivityReacts with: Mouse, Human
20 amino acid C-terminal peptide to Phosphoinositide 3 Kinase p110 delta
- ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Constituent: 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab1678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IP||Use at an assay dependent concentration. PubMed: 23023391|
|WB||1/2000 - 1/3000.|
Tissue specificityExpressed predominantly in leukocytes.
PathwayPhospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
Sequence similaritiesBelongs to the PI3/PI4-kinase family.
Contains 1 PI3K/PI4K domain.
modificationsAutophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity.
- Information by UniProt
- 5-bisphosphate 3-kinase 110 kDa catalytic subunit delta antibody
- 5-bisphosphate 3-kinase catalytic subunit delta isoform antibody
- APDS antibody
All lanes : Anti-PI 3 Kinase p110 delta antibody (ab1678) at 1/2000 dilution
Lane 1 : Peripheral Blood Mononuclear Cells (PBMCs)
Lane 2 : Primary human monocytes
Lane 3 : Primary human macropahges
Lane 4 : U937 monocytic cell line
Lane 5 : Whole human Lung
Lysates/proteins at 20 µg per lane.
All lanes : Bovine anti-Rabbit IgG-HRP at 1/2000 dilution
Developed using the ECL technique.
Exposure time: 1 minute
ICC/IF image of ab1678 stained HeLa (human epithelial cell line from cervix adenocarcinoma) cells. The cells were 4% formaldehyde fixed (10 minutes) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1678, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
This product has been referenced in:
- Shin YJ et al. PIP4K2A as a negative regulator of PI3K in PTEN-deficient glioblastoma. J Exp Med 216:1120-1134 (2019). Read more (PubMed: 30898893) »
- Goulielmaki E et al. Pharmacological inactivation of the PI3K p110d prevents breast tumour progression by targeting cancer cells and macrophages. Cell Death Dis 9:678 (2018). Read more (PubMed: 29880805) »