Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)

Submit a review Q&A (4)References (7)
Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)
  • Western blot - Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)
  • Western blot - Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)
  • Western blot - Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)
  • Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR386] to PI 3 Kinase p110 delta
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PI 3 Kinase p110 delta antibody [EPR386]
    See all PI 3 Kinase p110 delta primary antibodies

  • Description

    Rabbit monoclonal [EPR386] to PI 3 Kinase p110 delta

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human PI 3 Kinase p110 delta aa 200-300. The exact sequence is proprietary.

  • Positive control

    • WB: Jurkat, Raji, K562, RAW 264.7, Rat brain, Rat kidney and Rat spleen lysates. ICC/IF: RAW264.7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab109006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 119 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Tissue specificity

      Expressed predominantly in leukocytes.

    • Pathway

      Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.

    • Sequence similarities

      Belongs to the PI3/PI4-kinase family.
      Contains 1 PI3K/PI4K domain.

    • Post-translational
      modifications

      Autophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 5-bisphosphate 3-kinase 110 kDa catalytic subunit delta antibody
      • 5-bisphosphate 3-kinase catalytic subunit delta isoform antibody
      • APDS antibody
      • GRB1 antibody
      • IMD14 antibody
      • p110d antibody
      • p110delta antibody
      • p110dp85a antibody
      • p85-ALPHA antibody
      • Phosphatidylinositol 3 kinase catalytic delta polypeptide antibody
      • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit delta isoform antibody
      • Phosphatidylinositol 4,5 bisphosphate 3 kinase 110 kDa catalytic subunit delta antibody
      • Phosphatidylinositol 4,5 bisphosphate 3 kinase, catalytic subunit delta antibody
      • Phosphatidylinositol 45 bisphosphate 3 kinase catalytic subunit delta isoform antibody
      • Phosphatidylinositol-4 antibody
      • Phosphoinositide 3 kinase B antibody
      • Phosphoinositide 3 kinase C antibody
      • Phosphoinositide 3 kinase catalytic delta polypeptide antibody
      • Phosphoinositide 3 kinase, catalytic, delta polypeptide variant p37delta antibody
      • PI3 kinase p110 subunit delta antibody
      • PI3-kinase subunit delta antibody
      • PI3K antibody
      • PI3K-delta antibody
      • PI3Kdelta antibody
      • Pik3cd antibody
      • PIK3R1 antibody
      • PK3CD antibody
      • PK3CD_HUMAN antibody
      • PtdIns 3 kinase p110 antibody
      • PtdIns 3 kinase subunit p110 delta antibody
      • PtdIns-3-kinase subunit delta antibody
      • PtdIns-3-kinase subunit p110-delta antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)
      Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)

      Immunocytochemistry/ Immunofluorescence analysis of RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling PI 3 Kinase p110 delta with Purified ab109006 at 1:150 dilution (9.8 µg/ml). Cells were fixed in 100% Methanol and permeabilized with none. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor®488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Western blot - Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)
      Western blot - Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)
      All lanes : Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006) at 1.5 µg/ml (purified)

      Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
      Lane 2 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
      Lane 3 : Rat spleen lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 119 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

    • Western blot - Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)
      Western blot - Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)

      Lane 1: Wild type HAP1 whole cell lysate (40 µg)
      Lane 2: PIK3CD knockout HAP1 whole cell lysate (40 µg)
      Lane 3: Jurkat whole cell lysate (40 µg)
      Lane 4: K562 whole cell lysate (40 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109006 observed at 120 kDa. Red - loading control, ab8245, observed at 37 kDa.

      Unpurified ab109006 was shown to specifically react with PIK3CD in wild-type HAP1 cells. No band was observed when PIK3CD knockout samples were used. Wild-type and PIK3CD knockout samples were subjected to SDS-PAGE. Ab109006 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.

    • Western blot - Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)
      Western blot - Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)
      All lanes : Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006) at 1/1000 dilution (Unpurified)

      Lane 1 : Jurkat cell lysate

      Lane 2 : Raji cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : RAW 264.7 cell lysate
      Lane 5 : Rat brain tissue lysate
      Lane 6 : Rat kidney tissue lysate
      Lane 7 : Rat spleen tissue lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 119 kDa

    • Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)
      Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)

    References (7)

    Publishing research using ab109006? Please let us know so that we can cite the reference in this datasheet.

    ab109006 has been referenced in 7 publications.

    • Li Y  et al. Protective effect of CTRP6 on cerebral ischemia/reperfusion injury by attenuating inflammation, oxidative stress and apoptosis in PC12 cells. Mol Med Rep 22:344-352 (2020). PubMed: 32377750
    • Cui C  et al. Znhit1 inhibits breast cancer by up-regulating PTEN to deactivate the PI3K/Akt/mTOR pathway. Life Sci 224:204-211 (2019). PubMed: 30928405
    • Yao Y  et al. Knockdown of long noncoding RNA Malat1 aggravates hypoxia-induced cardiomyocyte injury by targeting miR-217. Adv Clin Exp Med 28:719-728 (2019). PubMed: 30843674
    • Li X  et al. Long non-coding RNA H19 knockdown inhibits the cell viability and promotes apoptosis of thyroid cancer cells through regulating the PI3K/AKT pathway. Exp Ther Med 18:1863-1869 (2019). PubMed: 31410148
    • Wang L  et al. Exosomes derived from pancreatic cancer cells induce insulin resistance in C2C12 myotube cells through the PI3K/Akt/FoxO1 pathway. Sci Rep 7:5384 (2017). PubMed: 28710412
    • Li Q  et al. Phosphoinositide 3-kinase p110d mediates estrogen- and FSH-stimulated ovarian follicle growth. Mol Endocrinol 27:1468-82 (2013). PubMed: 23820902
    • Le Floc'h A  et al. Annular PIP3 accumulation controls actin architecture and modulates cytotoxicity at the immunological synapse. J Exp Med 210:2721-37 (2013). PubMed: 24190432

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Question

    Thanks for the info.
    Do you also have the concentration of Lot#: GR42675-6?

    Read More
    Answer

    That lot is also 6.8 mg/ml. Any vial labeled GR42675 will be that concentration. The number after, for instance -6 or -7, is a reference number that identifies the date the antibody was aliquotted into the vial. Please let me know if you have other questions.

    Read More

    Question

    I recently purchased the PI3kd antibody (ab109006) and I noticed that
    it is in a tissue culture supernatant format with an unknown specific
    antibody concentration. What would be the appropriate negative
    isotype control and concentration for this antibody?

    Read More
    Answer

    Thank you for contacting us.

    The IgG concentration of the lot you have, GR42675-7, is 6.8 mg/ml.

    For an isotype control, I recommend antibody ab37415:

    Click here (or use the following: https://www.abcam.com/Rabbit-IgG-polyclonal-Isotype-Control-ab37415.html).

    The concentration of ab37415 is 5 mg/ml, so you will need to adjust the dilution factor to match the working concentration to that of ab109006.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    used ab32401 in Jurkat cells, saw no band
    received ab109006 as free of charge replacement

    for ab109006:
    Jurkat cells
    no band, but sees actin band
    Ab: 1/500
    secondary: OK
    looking for advice: use more protein, use more Ab, check if PI3K p85 can be detected

    Read More
    Answer

    Thank you for contacting us.
    I am sorry to hear that the antibodies for PI3K p110 delta do not seem to work as expected.

    As we discussed over the phone, I would like to suggest to try the following:

    1) Load moreprotein onto the gel.
    2) Use more primary antibody and incubate longer (e.g. overnight 4 degree - if you are not doing this already).
    3) If possible - check if PI3K p85 can be detected.

    Please let me know if these tips were of help. If not, please let me know as well.

    I wish you good luck and look forward to hear back from you.
    In the meantime, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    ab32401 is not detecting any bands via western blotting; secondary ab is ok; b-actin blot works well on same samples, used 1:500 with primary as suggested;

    Read More
    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab109006. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

    Read More

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