Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-PI 3 Kinase p110 delta antibody [EPR386] - BSA and Azide free (ab236049)

Overview

  • Product name

    Anti-PI 3 Kinase p110 delta antibody [EPR386] - BSA and Azide free
    See all PI 3 Kinase p110 delta primary antibodies
  • Description

    Rabbit monoclonal [EPR386] to PI 3 Kinase p110 delta - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PI 3 Kinase p110 delta aa 200-300. The exact sequence is proprietary.

  • Positive control

    • WB: HAP1, Jurkat and K562 whole cell lysates.
  • General notes

    Ab236049 is the carrier-free version of ab109006. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab236049 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab236049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 119 kDa.

Target

  • Tissue specificity

    Expressed predominantly in leukocytes.
  • Pathway

    Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
  • Sequence similarities

    Belongs to the PI3/PI4-kinase family.
    Contains 1 PI3K/PI4K domain.
  • Post-translational
    modifications

    Autophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5-bisphosphate 3-kinase 110 kDa catalytic subunit delta antibody
    • 5-bisphosphate 3-kinase catalytic subunit delta isoform antibody
    • APDS antibody
    • GRB1 antibody
    • IMD14 antibody
    • p110d antibody
    • p110delta antibody
    • p110dp85a antibody
    • p85-ALPHA antibody
    • Phosphatidylinositol 3 kinase catalytic delta polypeptide antibody
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit delta isoform antibody
    • Phosphatidylinositol 4,5 bisphosphate 3 kinase 110 kDa catalytic subunit delta antibody
    • Phosphatidylinositol 4,5 bisphosphate 3 kinase, catalytic subunit delta antibody
    • Phosphatidylinositol 45 bisphosphate 3 kinase catalytic subunit delta isoform antibody
    • Phosphatidylinositol-4 antibody
    • Phosphoinositide 3 kinase B antibody
    • Phosphoinositide 3 kinase C antibody
    • Phosphoinositide 3 kinase catalytic delta polypeptide antibody
    • Phosphoinositide 3 kinase, catalytic, delta polypeptide variant p37delta antibody
    • PI3 kinase p110 subunit delta antibody
    • PI3-kinase subunit delta antibody
    • PI3K antibody
    • PI3K-delta antibody
    • PI3Kdelta antibody
    • Pik3cd antibody
    • PIK3R1 antibody
    • PK3CD antibody
    • PK3CD_HUMAN antibody
    • PtdIns 3 kinase p110 antibody
    • PtdIns 3 kinase subunit p110 delta antibody
    • PtdIns-3-kinase subunit delta antibody
    • PtdIns-3-kinase subunit p110-delta antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence analysis of RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling PI 3 Kinase p110 delta with Purified ab109006 at 1:150 dilution (9.8 µg/ml). Cells were fixed in 100% Methanol and permeabilized with none. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109006).

  • Lane 1: Wild type HAP1 whole cell lysate (40 µg)
    Lane 2: PIK3CD knockout HAP1 whole cell lysate (40 µg)
    Lane 3: Jurkat whole cell lysate (40 µg)
    Lane 4: K562 whole cell lysate (40 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab109006 observed at 120 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab109006 was shown to specifically react with PIK3CD in wild-type HAP1 cells. No band was observed when PIK3CD knockout samples were used. Wild-type and PIK3CD knockout samples were subjected to SDS-PAGE. Ab109006 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109006).

References

ab236049 has not yet been referenced specifically in any publications.

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