Overview

  • Product name
    Anti-PI 3 Kinase p110 delta antibody [Y387]
    See all PI 3 Kinase p110 delta primary antibodies
  • Description
    Rabbit monoclonal [Y387] to PI 3 Kinase p110 delta
  • Host species
    Rabbit
  • Specificity
    ab32401 recognises PI3-Kinases (PI3-Ks).
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PI 3 Kinase p110 delta aa 250-350. The exact sequence is proprietary.

  • Positive control
    • Jurkat whole cell lysate (ab7899) and HeLa cells.
  • General notes

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 110 kDa (predicted molecular weight: 119 kDa).
ICC/IF 1/250 - 1/500.
IP 1/50.
  • Application notes
    Is unsuitable for Flow Cyt or IHC.
  • Target

    • Tissue specificity
      Expressed predominantly in leukocytes.
    • Pathway
      Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
    • Sequence similarities
      Belongs to the PI3/PI4-kinase family.
      Contains 1 PI3K/PI4K domain.
    • Post-translational
      modifications
      Autophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity.
    • Information by UniProt
    • Database links
    • Alternative names
      • 5-bisphosphate 3-kinase 110 kDa catalytic subunit delta antibody
      • 5-bisphosphate 3-kinase catalytic subunit delta isoform antibody
      • APDS antibody
      • GRB1 antibody
      • IMD14 antibody
      • p110d antibody
      • p110delta antibody
      • p110dp85a antibody
      • p85-ALPHA antibody
      • Phosphatidylinositol 3 kinase catalytic delta polypeptide antibody
      • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit delta isoform antibody
      • Phosphatidylinositol 4,5 bisphosphate 3 kinase 110 kDa catalytic subunit delta antibody
      • Phosphatidylinositol 4,5 bisphosphate 3 kinase, catalytic subunit delta antibody
      • Phosphatidylinositol 45 bisphosphate 3 kinase catalytic subunit delta isoform antibody
      • Phosphatidylinositol-4 antibody
      • Phosphoinositide 3 kinase B antibody
      • Phosphoinositide 3 kinase C antibody
      • Phosphoinositide 3 kinase catalytic delta polypeptide antibody
      • Phosphoinositide 3 kinase, catalytic, delta polypeptide variant p37delta antibody
      • PI3 kinase p110 subunit delta antibody
      • PI3-kinase subunit delta antibody
      • PI3K antibody
      • PI3K-delta antibody
      • PI3Kdelta antibody
      • Pik3cd antibody
      • PIK3R1 antibody
      • PK3CD antibody
      • PK3CD_HUMAN antibody
      • PtdIns 3 kinase p110 antibody
      • PtdIns 3 kinase subunit p110 delta antibody
      • PtdIns-3-kinase subunit delta antibody
      • PtdIns-3-kinase subunit p110-delta antibody
      see all

    Images

    • Anti-PI 3 Kinase p110 delta antibody [Y387] (ab32401) at 1/500 dilution + Jurkat cell lysate

      Predicted band size: 119 kDa
      Observed band size: 110 kDa
      why is the actual band size different from the predicted?

    • Immunofluorescent staining of HeLa cells using ab32401 at 1/250 dilution.

    References

    This product has been referenced in:
    • Milara J  et al. Non-neuronal cholinergic system contributes to corticosteroid resistance in chronic obstructive pulmonary disease patients. Respir Res 17:145 (2016). IP ; Human . Read more (PubMed: 27825347) »
    • Mendizabal-Zubiaga J  et al. Cannabinoid CB1 Receptors Are Localized in Striated Muscle Mitochondria and Regulate Mitochondrial Respiration. Front Physiol 7:476 (2016). IP ; Mouse . Read more (PubMed: 27826249) »
    See all 11 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for contacting us.
    I am sorry to hear that the antibodies for PI3K p110 delta do not seem to work as expected.

    As we discussed over the phone, I would like to suggest to try the following:

    1) Load moreprotein onto the gel.
    2) Use more primary antibody and incubate longer (e.g. overnight 4 degree - if you are not doing this already).
    3) If possible - check if PI3K p85 can be detected.

    Please let me know if these tips were of help. If not, please let me know as well.

    I wish you good luck and look forward to hear back from you.
    In the meantime, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab109006. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up