Overview

  • Product name

    Anti-PI 3 Kinase p55 gamma antibody
    See all PI 3 Kinase p55 gamma primary antibodies
  • Description

    Goat polyclonal to PI 3 Kinase p55 gamma
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • WB: PI 3 Kinase p55 gamma overexpressing HEK-293T whole cell lysate; mouse testis lysate. IHC-P: Human tonsil tissue. ICC/IF: A431 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab235234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 54 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 15 µg/ml.

Target

  • Function

    Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.
  • Tissue specificity

    Highest levels in brain and testis. Lower levels in adipose tissue, kidney, heart, lung and skeletal muscle.
  • Sequence similarities

    Belongs to the PI3K p85 subunit family.
    Contains 2 SH2 domains.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp686P05226 antibody
    • FLJ41892 antibody
    • OTTHUMP00000009783 antibody
    • OTTHUMP00000009786 antibody
    • p55 antibody
    • p55 gamma antibody
    • P55G_HUMAN antibody
    • p55PIK antibody
    • Phosphatidylinositol 3 kinase regulatory subunit gamma antibody
    • Phosphatidylinositol 3 kinase regulatory subunit polypeptide 3 antibody
    • Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 (p55, gamma) antibody
    • Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma antibody
    • Phosphatidylinositol 3-kinase regulatory subunit gamma antibody
    • Phosphoinositide 3 kinase regulatory subunit 3 (gamma) antibody
    • Phosphoinositide 3 kinase regulatory subunit 3 antibody
    • Phosphoinositide 3 kinase regulatory subunit polypeptide 3 antibody
    • Phosphoinositide 3 kinase, regulatory subunit 3 (p55, gamma) antibody
    • Phosphoinositide 3 kinase, regulatory subunit, polypeptide 3 (p55, gamma) antibody
    • PI3 kinase p85 subunit gamma antibody
    • PI3-kinase regulatory subunit gamma antibody
    • PI3-kinase subunit p55-gamma antibody
    • PI3K regulatory subunit gamma antibody
    • Pik3r3 antibody
    • PtdIns 3 kinase p85 gamma antibody
    • PtdIns-3-kinase regulatory subunit gamma antibody
    • PtdIns-3-kinase regulatory subunit p55-gamma antibody
    see all

Images

  • Anti-PI 3 Kinase p55 gamma antibody (ab235234) at 1/1000 dilution + Mouse testis lysate

    Secondary
    Goat IgG-HRP at 1/5000 dilution

    Predicted band size: 54 kDa


    Exposure time: 1 second
  • All lanes : Anti-PI 3 Kinase p55 gamma antibody (ab235234) at 1/1000 dilution

    Lane 1 : PI 3 Kinase P55 gamma overexpressing HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : Empty vector HEK-293T lysate

    Secondary
    All lanes : Goat IgG HRP at 1/40000 dilution

    Predicted band size: 54 kDa


    Exposure time: 75 seconds
  • Paraffin-embedded human tonsil tissue stained for PI 3 Kinase p55 gamma using ab235234 at 1/100 dilution for 30 minutes at room temperature, in immunohistochemical analysis. Secondary antibody was Donkey anti-Goat (HRP) (Ready to use). Counterstained with hematoxylin.

    HIER using Citrate Buffer for 20min.

    Panel A) Human testis H&E stain.
    Panel B) ab235234 staining at 20X.
    Panel C) ab235234 staining at 40X.

  • Paraffin-embedded human tonsil tissue stained for PI 3 Kinase p55 gamma using ab235234 at 1/100 dilution for 30 minutes at room temperature, in immunohistochemical analysis. Secondary antibody was Donkey anti-Goat (HRP) 4 µg/ml for 20 minutes at room temperature. Counterstained with hematoxylin.

    HIER using Citrate Buffer for 20min.

    Panel A) Human tonsil H&E stain.
    Panel B) ab235234 staining at 20X.
    Panel C) ab235234 staining at 40X.

  • 100% methanol-fixed, 0.3% Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for PI 3 Kinase p55 gamma (Green) using ab235234 at 15 µg/ml overnight at 4°C. The secondary antibody was Donkey anti-Goat IgG DiLight™ 488 Conjugated antibody at 15 µg/ml for 1 hour at room temperature. The nuclear counterstain was DAPI (Blue).

    (A) DAPI               

    (B) ab235234 + Secondary            

    (C) Merge A+B 

    (D) Secondary only

    Expected localization: Cytosol (UniProt), Nucleoplams (HPA) – distinct punctate staining in nucleus could indicate nucleoplasm.

References

ab235234 has not yet been referenced specifically in any publications.

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