Key features and details
- Rabbit monoclonal [NO.4F10A1] to PI 3 Kinase p55 gamma
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PI 3 Kinase p55 gamma antibody [NO.4F10A1]
See all PI 3 Kinase p55 gamma primary antibodies
DescriptionRabbit monoclonal [NO.4F10A1] to PI 3 Kinase p55 gamma
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
- WB: MCF7 and Jurkat cell extracts; mouse testis extract. IHC-P: Mouse brain and testis tissue. ICC/IF: SH-SY5Y and PC-3M cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
Our Abpromise guarantee covers the use of ab238509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/1000. Predicted molecular weight: 54 kDa.|
|IHC-P||1/50 - 1/200.|
FunctionBinds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.
Tissue specificityHighest levels in brain and testis. Lower levels in adipose tissue, kidney, heart, lung and skeletal muscle.
Sequence similaritiesBelongs to the PI3K p85 subunit family.
Contains 2 SH2 domains.
- Information by UniProt
- DKFZp686P05226 antibody
- FLJ41892 antibody
- OTTHUMP00000009783 antibody
All lanes : Anti-PI 3 Kinase p55 gamma antibody [NO.4F10A1] (ab238509) at 1/500 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell extract
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
Lane 3 : Mouse testis extract
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 54 kDa
Blocking buffer: 3% non-fat dry milk in TBST.
Paraffin-embedded mouse brain tissue stained for PI 3 Kinase p55 gamma using ab238509 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded mouse testis tissue stained for PI 3 Kinase p55 gamma using ab238509 at 1/100 dilution in immunohistochemical analysis.
SH-SY5Y (human neuroblastoma cell line from bone marrow) cells stained for PI 3 Kinase p55 gamma (green) using ab238509 at 1/50 dilution in ICC/IF.
PC-3M (human prostate adenocarcinoma cell line) cells stained for PI 3 Kinase p55 gamma (green) using ab238509 at 1/50 dilution in ICC/IF.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238509 has been referenced in 1 publication.
- Molinaro A et al. Insulin-Driven PI3K-AKT Signaling in the Hepatocyte Is Mediated by Redundant PI3Ka and PI3Kß Activities and Is Promoted by RAS. Cell Metab 29:1400-1409.e5 (2019). PubMed: 30982732