Overview

  • Product name

    Anti-PI 3 Kinase p55 gamma antibody [NO.4F10A1]
    See all PI 3 Kinase p55 gamma primary antibodies
  • Description

    Rabbit monoclonal [NO.4F10A1] to PI 3 Kinase p55 gamma
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human PI 3 Kinase p55 gamma aa 278-297.
    Sequence:

    EQDLKNQALDNREIDKKMNS


    Database link: Q92569

  • Positive control

    • WB: MCF7 and Jurkat cell extracts; mouse testis extract. IHC-P: Mouse brain and testis tissue. ICC/IF: SH-SY5Y and PC-3M cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    NO.4F10A1
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab238509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
WB 1/500 - 1/1000. Predicted molecular weight: 54 kDa.
IHC-P 1/50 - 1/200.

Target

  • Function

    Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.
  • Tissue specificity

    Highest levels in brain and testis. Lower levels in adipose tissue, kidney, heart, lung and skeletal muscle.
  • Sequence similarities

    Belongs to the PI3K p85 subunit family.
    Contains 2 SH2 domains.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp686P05226 antibody
    • FLJ41892 antibody
    • OTTHUMP00000009783 antibody
    • OTTHUMP00000009786 antibody
    • p55 antibody
    • p55 gamma antibody
    • P55G_HUMAN antibody
    • p55PIK antibody
    • Phosphatidylinositol 3 kinase regulatory subunit gamma antibody
    • Phosphatidylinositol 3 kinase regulatory subunit polypeptide 3 antibody
    • Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 (p55, gamma) antibody
    • Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma antibody
    • Phosphatidylinositol 3-kinase regulatory subunit gamma antibody
    • Phosphoinositide 3 kinase regulatory subunit 3 (gamma) antibody
    • Phosphoinositide 3 kinase regulatory subunit 3 antibody
    • Phosphoinositide 3 kinase regulatory subunit polypeptide 3 antibody
    • Phosphoinositide 3 kinase, regulatory subunit 3 (p55, gamma) antibody
    • Phosphoinositide 3 kinase, regulatory subunit, polypeptide 3 (p55, gamma) antibody
    • PI3 kinase p85 subunit gamma antibody
    • PI3-kinase regulatory subunit gamma antibody
    • PI3-kinase subunit p55-gamma antibody
    • PI3K regulatory subunit gamma antibody
    • Pik3r3 antibody
    • PtdIns 3 kinase p85 gamma antibody
    • PtdIns-3-kinase regulatory subunit gamma antibody
    • PtdIns-3-kinase regulatory subunit p55-gamma antibody
    see all

Images

  • All lanes : Anti-PI 3 Kinase p55 gamma antibody [NO.4F10A1] (ab238509) at 1/500 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell extract
    Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
    Lane 3 : Mouse testis extract

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size: 54 kDa



    Blocking buffer: 3% non-fat dry milk in TBST.

  • Paraffin-embedded mouse brain tissue stained for PI 3 Kinase p55 gamma using ab238509 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse testis tissue stained for PI 3 Kinase p55 gamma using ab238509 at 1/100 dilution in immunohistochemical analysis.

  • SH-SY5Y (human neuroblastoma cell line from bone marrow) cells stained for PI 3 Kinase p55 gamma (green) using ab238509 at 1/50 dilution in ICC/IF.

  • PC-3M (human prostate adenocarcinoma cell line) cells stained for PI 3 Kinase p55 gamma (green) using ab238509 at 1/50 dilution in ICC/IF.

References

This product has been referenced in:

  • Molinaro A  et al. Insulin-Driven PI3K-AKT Signaling in the Hepatocyte Is Mediated by Redundant PI3Ka and PI3Kß Activities and Is Promoted by RAS. Cell Metab 29:1400-1409.e5 (2019). Read more (PubMed: 30982732) »
See 1 Publication for this product

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