Overview

  • Product name

    Anti-PI 3 Kinase p85 alpha antibody [6G10]
    See all PI 3 Kinase p85 alpha primary antibodies
  • Description

    Mouse monoclonal [6G10] to PI 3 Kinase p85 alpha
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Purified recombinant fragment of Human PI 3 Kinase p85 alpha (amino acids 159-388) expressed in E.coli

  • Positive control

    • Human PI 3 Kinase p85 alpha recombinant protein; HeLa, HepG2 and NIH3T3 cells; cervical cancer and rectum cancer tissues.
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR130547-5 are from Tissue Culture Supernatant

Properties

Applications

Our Abpromise guarantee covers the use of ab135253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 83.6 kDa.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100.

Target

  • Function

    Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
  • Tissue specificity

    Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).
  • Sequence similarities

    Belongs to the PI3K p85 subunit family.
    Contains 1 Rho-GAP domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • Domain

    The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.
  • Post-translational
    modifications

    Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
    Phosphorylated. Dephosphorylated by PTPRJ.
  • Information by UniProt
  • Database links

  • Alternative names

    • GRB1 antibody
    • p85 alpha antibody
    • p85 antibody
    • P85A_HUMAN antibody
    • Phosphatidylinositol 3 kinase associated p 85 alpha antibody
    • Phosphatidylinositol 3 kinase regulatory 1 antibody
    • Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 1 (p85 alpha) antibody
    • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody
    • Phosphatidylinositol 3-kinase regulatory subunit alpha antibody
    • Phosphoinositide 3 kinase, regulatory subunit 1 (alpha) antibody
    • PI3 kinase p85 subunit alpha antibody
    • PI3-kinase regulatory subunit alpha antibody
    • PI3-kinase subunit p85-alpha antibody
    • PI3K antibody
    • PI3K regulatory subunit alpha antibody
    • Pik3r1 antibody
    • PtdIns 3 kinase p85 alpha antibody
    • PtdIns-3-kinase regulatory subunit alpha antibody
    • PtdIns-3-kinase regulatory subunit p85-alpha antibody
    see all

Images

  • Anti-PI 3 Kinase p85 alpha antibody [6G10] (ab135253) at 1/500 dilution + Human PI 3 Kinase p85 alpha recombinant protein.

    Predicted band size: 83.6 kDa



    Note: the predicted band size of the full length protein is 83.6 kDa. The expected band size of the recombinant fragment used here is 53.4 kDa.

  • Immunofluorescence analysis of HeLa cells using ab135253 at 1/100 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Immunofluorescence analysis of HepG2 cells using ab135253 at 1/100 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Flow cytometric analysis of NIH3T3 cells using ab135253 at 1/200 dilution (green) and negative control (red).
  • Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ab135253 at 1/200 dilution with DAB staining.
  • Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ab135253 at 1/200 dilution with DAB staining.

References

This product has been referenced in:

  • Goretsky T  et al. A Cytosolic Multiprotein Complex Containing p85a Is Required for ß-Catenin Activation in Colitis and Colitis-associated Cancer. J Biol Chem 291:4166-77 (2016). Read more (PubMed: 26565021) »
See 1 Publication for this product

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