Product nameAnti-PI 3 Kinase p85 alpha antibody
See all PI 3 Kinase p85 alpha primary antibodies
DescriptionRabbit polyclonal to PI 3 Kinase p85 alpha
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Zebrafish, Rhesus monkey
Synthetic peptide within Human PI 3 Kinase p85 alpha (internal sequence). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: P27986
- WB: Jurkat and Raji whole cell extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226826 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 84 kDa.|
FunctionBinds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
Tissue specificityIsoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).
Sequence similaritiesBelongs to the PI3K p85 subunit family.
Contains 1 Rho-GAP domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain.
DomainThe SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.
modificationsPolyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
Phosphorylated. Dephosphorylated by PTPRJ.
- Information by UniProt
- GRB1 antibody
- p85 alpha antibody
- p85 antibody
All lanes : Anti-PI 3 Kinase p85 alpha antibody (ab226826) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 84 kDa
7.5% SDS-PAGE gel.
ab226826 has not yet been referenced specifically in any publications.