Overview

  • Product name

    Anti-PI 3 Kinase p85 alpha antibody
    See all PI 3 Kinase p85 alpha primary antibodies
  • Description

    Rabbit polyclonal to PI 3 Kinase p85 alpha
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human PI 3 Kinase p85 alpha (internal sequence). The exact sequence is proprietary.
    Database link: P27986

  • Positive control

    • WB: PC-12, Rat2, Neuro-2a, C8D30, NIH/3T3, Raw 264.7, C2C12, Jurkat, K562 and THP-1 whole cell extracts. IP: Jurkat whole cell extract. IHC-P: Mouse duodenum tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab227204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 83 kDa.
IP 1/100 - 1/500.

Target

  • Function

    Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
  • Tissue specificity

    Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).
  • Sequence similarities

    Belongs to the PI3K p85 subunit family.
    Contains 1 Rho-GAP domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • Domain

    The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.
  • Post-translational
    modifications

    Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
    Phosphorylated. Dephosphorylated by PTPRJ.
  • Information by UniProt
  • Database links

  • Alternative names

    • GRB1 antibody
    • p85 alpha antibody
    • p85 antibody
    • P85A_HUMAN antibody
    • Phosphatidylinositol 3 kinase associated p 85 alpha antibody
    • Phosphatidylinositol 3 kinase regulatory 1 antibody
    • Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 1 (p85 alpha) antibody
    • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody
    • Phosphatidylinositol 3-kinase regulatory subunit alpha antibody
    • Phosphoinositide 3 kinase, regulatory subunit 1 (alpha) antibody
    • PI3 kinase p85 antibody
    • PI3 kinase p85 subunit alpha antibody
    • PI3-kinase regulatory subunit alpha antibody
    • PI3-kinase subunit p85-alpha antibody
    • PI3K antibody
    • PI3K p85 antibody
    • PI3K regulatory subunit alpha antibody
    • Pik3r1 antibody
    • PtdIns 3 kinase p85 alpha antibody
    • PtdIns-3-kinase regulatory subunit alpha antibody
    • PtdIns-3-kinase regulatory subunit p85-alpha antibody
    see all

Images

  • All lanes : Anti-PI 3 Kinase p85 alpha antibody (ab227204) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
    Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell extract
    Lane 3 : THP-1 (human monocytic leukemia cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 83 kDa



    7.5% SDS-PAGE

  • Paraffin-embedded mouse duodenum stained for PI 3 Kinase p85 alpha with ab227204 at 1/500 dilution in immunohistochemical analysis.

  • PI 3 Kinase p85 alpha was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract with 5 µg ab227204. Western blot was performed from the immunoprecipitate using ab227204 at 1/500 dilution.

    Lane 1: Control IgG IP in Jurkat whole cell extract.

    Lane 2: ab227204 IP in Jurkat whole cell extract.

    10% SDS-PAGE.

  • All lanes : Anti-PI 3 Kinase p85 alpha antibody (ab227204) at 1/500 dilution

    Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
    Lane 2 : C8D30 whole cell extract
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
    Lane 4 : Raw 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
    Lane 5 : C2C12 (mouse myoblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 83 kDa



    7.5% SDS-PAGE

  • All lanes : Anti-PI 3 Kinase p85 alpha antibody (ab227204) at 1/1000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
    Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 83 kDa



    7.5% SDS-PAGE

References

This product has been referenced in:

  • Wang X  et al. Upregulation of lncRNA FER1L4 suppresses the proliferation and migration of the hepatocellular carcinoma via regulating PI3K/AKT signal pathway. J Cell Biochem N/A:N/A (2018). Read more (PubMed: 30382631) »
  • Bao J  et al. Fmr1 protects cardiomyocytes against lipopolysaccharide-induced myocardial injury. Exp Ther Med 16:1825-1833 (2018). Read more (PubMed: 30186407) »
See all 2 Publications for this product

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