Overview

  • Product name

    Anti-PI 3 Kinase p85 alpha antibody [EPR5513]
    See all PI 3 Kinase p85 alpha primary antibodies
  • Description

    Rabbit monoclonal [EPR5513] to PI 3 Kinase p85 alpha
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminus of Human PI 3 Kinase p85 alpha (UniProt ID: P27986)

  • Positive control

    • Jurkat, Raji, K562, MCF7, C6, Raw 264.7, PC12, NIH3T3 cell lysates
  • General notes

    A trial size is available to purchase for this antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 83 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
    • Tissue specificity

      Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).
    • Sequence similarities

      Belongs to the PI3K p85 subunit family.
      Contains 1 Rho-GAP domain.
      Contains 2 SH2 domains.
      Contains 1 SH3 domain.
    • Domain

      The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.
    • Post-translational
      modifications

      Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
      Phosphorylated. Dephosphorylated by PTPRJ.
    • Information by UniProt
    • Database links

    • Alternative names

      • GRB1 antibody
      • p85 alpha antibody
      • p85 antibody
      • P85A_HUMAN antibody
      • Phosphatidylinositol 3 kinase associated p 85 alpha antibody
      • Phosphatidylinositol 3 kinase regulatory 1 antibody
      • Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 1 (p85 alpha) antibody
      • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody
      • Phosphatidylinositol 3-kinase regulatory subunit alpha antibody
      • Phosphoinositide 3 kinase, regulatory subunit 1 (alpha) antibody
      • PI3 kinase p85 subunit alpha antibody
      • PI3-kinase regulatory subunit alpha antibody
      • PI3-kinase subunit p85-alpha antibody
      • PI3K antibody
      • PI3K regulatory subunit alpha antibody
      • Pik3r1 antibody
      • PtdIns 3 kinase p85 alpha antibody
      • PtdIns-3-kinase regulatory subunit alpha antibody
      • PtdIns-3-kinase regulatory subunit p85-alpha antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: PI 3 Kinase p85 alpha knockout HAP1 cell lysate (20 µg)
      Lane 3: Jurkat cell lysate (20 µg)
      Lane 4: THP1 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab133595 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab133595 was shown to specifically react with PI 3 Kinase p85 alpha when PI 3 Kinase p85 alpha knockout samples were used. Wild-type and PI 3 Kinase p85 alpha knockout samples were subjected to SDS-PAGE. ab133595 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-PI 3 Kinase p85 alpha antibody [EPR5513] (ab133595) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : MCF7 cell lysate
      Lane 5 : C6 cell lysate
      Lane 6 : Raw 264.7 cell lysate
      Lane 7 : PC12 cell lysate
      Lane 8 : NIH3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti Rabbit at 1/2000 dilution

      Predicted band size: 83 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Molinaro A  et al. Insulin-Driven PI3K-AKT Signaling in the Hepatocyte Is Mediated by Redundant PI3Ka and PI3Kß Activities and Is Promoted by RAS. Cell Metab 29:1400-1409.e5 (2019). Read more (PubMed: 30982732) »
    • Zhu B  et al. Downregulation of Krüppel-like factor 1 inhibits the metastasis and invasion of cervical cancer cells. Mol Med Rep 18:3932-3940 (2018). Read more (PubMed: 30132534) »
    See all 4 Publications for this product

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