Product nameAnti-PI 3 Kinase p85 alpha antibody [M253]
See all PI 3 Kinase p85 alpha primary antibodies
DescriptionMouse monoclonal [M253] to PI 3 Kinase p85 alpha
Tested applicationsSuitable for: WB, ICC, IHC-P, ICC/IF, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken
Recombinant fragment corresponding to Human PI 3 Kinase p85 alpha (C terminal).
- A431 or Jurkat whole cell lysate (ab7899). This antibody gave a positive result in IHC in the following FFPE tissue: Human skeletal muscle.
Phosphoinositide 3-kinase (PI3K) phosphorylates phosphatidylinositol (PI), PI-4-phosphate and PI-4,5-bisphosphate to catalyze the production of PI-3,4,5-triphosphate. Growth factors and hormones activate PI 3 Kinase to coordinate various cellular events, such as cell growth, cell cycle entry, cell migration and cell survival.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, 49% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab86714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).|
|IHC-P||Use a concentration of 5 µg/ml.|
|ICC/IF||Use at an assay dependent concentration.|
Only tested in Peptide ELISA
FunctionBinds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
Tissue specificityIsoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).
Sequence similaritiesBelongs to the PI3K p85 subunit family.
Contains 1 Rho-GAP domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain.
DomainThe SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.
modificationsPolyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
Phosphorylated. Dephosphorylated by PTPRJ.
- Information by UniProt
- GRB1 antibody
- p85 alpha antibody
- p85 antibody
Lane 1 : Anti-PI 3 Kinase p85 alpha antibody [M253] (ab86714) at 1/1000 dilution
Lane 2 : Anti-PI 3 Kinase p85 alpha antibody [M253] (ab86714) at 1/2000 dilution
Lane 3 : Anti-PI 3 Kinase p85 alpha antibody [M253] (ab86714) at 1/4000 dilution
All lanes : A431 cell lysate
Predicted band size: 85 kDa
Observed band size: 85 kDa
Immunocytochemical labeling of PI 3 Kinase p85 in aldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with ab86714, then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.
IHC image of PI 3 Kinase p85 staining in Human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (ab64236) (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86714, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ICC/IF image of ab86714 stained SKNSH cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86714, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) (ab96871) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Li ZR et al. SOX2 knockdown inhibits the migration and invasion of basal cell carcinoma cells by targeting the SRPK1-mediated PI3K/AKT signaling pathway. Oncol Lett 17:1617-1625 (2019). Read more (PubMed: 30675221) »
- Zhang X et al. Tanshinone IIA induces apoptosis of ovarian cancer cells in vitro and in vivo through attenuation of PI3K/AKT/JNK signaling pathways. Oncol Lett 17:1896-1902 (2019). Read more (PubMed: 30675253) »