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Signal Transduction Signaling Pathway Lipid Signaling Lipid Kinases
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Anti-PI 3 Kinase p85 alpha antibody - N-terminal (ab231469)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody - N-terminal (ab231469)

    Key features and details

    • Rabbit polyclonal to PI 3 Kinase p85 alpha - N-terminal
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

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    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
    Product image
    Recombinant Human PI 3 Kinase p85 alpha protein (ab84769)

    View more associated products

    Overview

    • Product name

      Anti-PI 3 Kinase p85 alpha antibody - N-terminal
      See all PI 3 Kinase p85 alpha primary antibodies
    • Description

      Rabbit polyclonal to PI 3 Kinase p85 alpha - N-terminal
    • Host species

      Rabbit
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Rabbit, Cow, Frog
    • Immunogen

      Synthetic peptide within Human PI 3 Kinase p85 alpha aa 1-100 (N terminal). The exact sequence is proprietary.
      Database link: P27986

    • Positive control

      • IHC-P: Human breast carcinoma tissue.
    • General notes

      This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.60
      Preservative: 0.1% Sodium azide
      Constituents: PBS, 1% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Signaling Pathway
      • Lipid Signaling
      • Lipid Kinases
      • Cancer
      • Signal transduction
      • Other
      • Immunology
      • Innate Immunity
      • TLR Signaling

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human PI 3 Kinase p85 alpha protein (ab84769)
    • Related Products

      • Recombinant Human PI 3 Kinase p85 alpha protein (ab123752)
      • Recombinant Human PI 3 Kinase p85 alpha protein (ab84769)

    Applications

    Our Abpromise guarantee covers the use of ab231469 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P 1/50.

    Boil tissue section in Citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.

    Incubate with primary antibody for 10 minutes at room temperature.

    Target

    • Function

      Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
    • Tissue specificity

      Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).
    • Sequence similarities

      Belongs to the PI3K p85 subunit family.
      Contains 1 Rho-GAP domain.
      Contains 2 SH2 domains.
      Contains 1 SH3 domain.
    • Domain

      The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.
    • Post-translational
      modifications

      Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
      Phosphorylated. Dephosphorylated by PTPRJ.
    • Target information above from: UniProt accession P27986 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 282307 Cow
      • Entrez Gene: 5295 Human
      • Entrez Gene: 18708 Mouse
      • Entrez Gene: 25513 Rat
      • Omim: 171833 Human
      • SwissProt: P23727 Cow
      • SwissProt: P27986 Human
      • SwissProt: P26450 Mouse
      • SwissProt: Q63787 Rat
      • Unigene: 132225 Human
      • Unigene: 604502 Human
      • Unigene: 259333 Mouse
      • Unigene: 10599 Rat
      see all
    • Alternative names

      • GRB1 antibody
      • p85 alpha antibody
      • p85 antibody
      • P85A_HUMAN antibody
      • Phosphatidylinositol 3 kinase associated p 85 alpha antibody
      • Phosphatidylinositol 3 kinase regulatory 1 antibody
      • Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 1 (p85 alpha) antibody
      • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody
      • Phosphatidylinositol 3-kinase regulatory subunit alpha antibody
      • Phosphoinositide 3 kinase, regulatory subunit 1 (alpha) antibody
      • PI3 kinase p85 antibody
      • PI3 kinase p85 subunit alpha antibody
      • PI3-kinase regulatory subunit alpha antibody
      • PI3-kinase subunit p85-alpha antibody
      • PI3K antibody
      • PI3K p85 antibody
      • PI3K regulatory subunit alpha antibody
      • Pik3r1 antibody
      • PtdIns 3 kinase p85 alpha antibody
      • PtdIns-3-kinase regulatory subunit alpha antibody
      • PtdIns-3-kinase regulatory subunit p85-alpha antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody - N-terminal (ab231469)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody - N-terminal (ab231469)

      Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for PI 3 Kinase p85 using ab231469 at 1/50 dilution in immunohistochemical analysis.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab231469? Please let us know so that we can cite the reference in this datasheet.

    ab231469 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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