Recombinant
RabMAb

Recombinant Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

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Overview

  • Product name

    Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free
    See all PI 3 Kinase p85 alpha primary antibodies

  • Description

    Rabbit monoclonal [SP62] to PI 3 Kinase p85 alpha - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow

  • Immunogen

    Synthetic peptide within Human PI 3 Kinase p85 alpha aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P27986

  • Positive control

    • IHC-P: Human placenta tissue ICC/IF: NIH/3T3 and MCF7 cells FC: PC-12, NIH/3T3 and MCF7 cells

  • General notes

    Ab236440 is the carrier-free version of ab225720. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab236440 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Applications

Our Abpromise guarantee covers the use of ab236440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Primary incubation for 30 minutes at 4°C.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary incubation for 30 minutes at room temperature.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

  • Tissue specificity

    Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).

  • Sequence similarities

    Belongs to the PI3K p85 subunit family.
    Contains 1 Rho-GAP domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.

  • Domain

    The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.

  • Post-translational
    modifications

    Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
    Phosphorylated. Dephosphorylated by PTPRJ.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • GRB1 antibody
    • p85 alpha antibody
    • p85 antibody
    • P85A_HUMAN antibody
    • Phosphatidylinositol 3 kinase associated p 85 alpha antibody
    • Phosphatidylinositol 3 kinase regulatory 1 antibody
    • Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 1 (p85 alpha) antibody
    • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody
    • Phosphatidylinositol 3-kinase regulatory subunit alpha antibody
    • Phosphoinositide 3 kinase, regulatory subunit 1 (alpha) antibody
    • PI3 kinase p85 antibody
    • PI3 kinase p85 subunit alpha antibody
    • PI3-kinase regulatory subunit alpha antibody
    • PI3-kinase subunit p85-alpha antibody
    • PI3K antibody
    • PI3K p85 antibody
    • PI3K regulatory subunit alpha antibody
    • Pik3r1 antibody
    • PtdIns 3 kinase p85 alpha antibody
    • PtdIns-3-kinase regulatory subunit alpha antibody
    • PtdIns-3-kinase regulatory subunit p85-alpha antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling PI 3 Kinase p85 alpha with purified ab225720 at 1/250 (10 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Flow cytometry analysis of MCF-7 (human breast carcinoma) labeling PI 3 Kinase p85 alpha with purified ab225720 at 1/250 dilution (10.32 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (mouse embryonic fibroblast) cells labeling PI 3 Kinase p85 alpha with purified ab225720 at 1/250 (10 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Flow cytometry analysis of PC-12 (rat adrenal gland pheochromocytoma) labeling PI 3 Kinase p85 alpha with purified ab225720 at 1/2500 dilution (1.03 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Flow cytometry analysis of NIH/3T3 (mouse embryo) labeling PI 3 Kinase p85 alpha with purified ab225720 at 1/250 dilution (10.32 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Flow cytometric analysis of MCF7 (human breast adenocarcinoma cell line) cell line labeling PI 3 Kinase p85 alpha with ab225720 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human melanoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human testis tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human breast adenocarcinoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human placenta tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

References

ab236440 has not yet been referenced specifically in any publications.

Publishing research using ab236440? Please let us know so that we can cite the reference in this datasheet.

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