RecombinantRabMAb

Recombinant Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

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Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
  • Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
  • Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
  • Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
  • Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
  • Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
  • Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP62] to PI 3 Kinase p85 alpha - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free
    See all PI 3 Kinase p85 alpha primary antibodies

  • Description

    Rabbit monoclonal [SP62] to PI 3 Kinase p85 alpha - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow

  • Immunogen

    Synthetic peptide within Human PI 3 Kinase p85 alpha aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P27986

  • Positive control

    • IHC-P: Human placenta tissue ICC/IF: NIH/3T3 and MCF7 cells Flow Cyt: PC-12, NIH/3T3 and MCF7 cells

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    Ab236440 is the carrier-free version of ab225720. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab236440 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab236440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Primary incubation for 30 minutes at 4°C.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary incubation for 30 minutes at room temperature.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

  • Tissue specificity

    Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).

  • Sequence similarities

    Belongs to the PI3K p85 subunit family.
    Contains 1 Rho-GAP domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.

  • Domain

    The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.

  • Post-translational
    modifications

    Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
    Phosphorylated. Dephosphorylated by PTPRJ.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • GRB1 antibody
    • p85 alpha antibody
    • p85 antibody
    • P85A_HUMAN antibody
    • Phosphatidylinositol 3 kinase associated p 85 alpha antibody
    • Phosphatidylinositol 3 kinase regulatory 1 antibody
    • Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 1 (p85 alpha) antibody
    • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody
    • Phosphatidylinositol 3-kinase regulatory subunit alpha antibody
    • Phosphoinositide 3 kinase, regulatory subunit 1 (alpha) antibody
    • PI3 kinase p85 antibody
    • PI3 kinase p85 subunit alpha antibody
    • PI3-kinase regulatory subunit alpha antibody
    • PI3-kinase subunit p85-alpha antibody
    • PI3K antibody
    • PI3K p85 antibody
    • PI3K regulatory subunit alpha antibody
    • Pik3r1 antibody
    • PtdIns 3 kinase p85 alpha antibody
    • PtdIns-3-kinase regulatory subunit alpha antibody
    • PtdIns-3-kinase regulatory subunit p85-alpha antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling PI 3 Kinase p85 alpha with purified ab225720 at 1/250 (10 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Flow cytometry analysis of MCF-7 (human breast carcinoma) labeling PI 3 Kinase p85 alpha with purified ab225720 at 1/250 dilution (10.32 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (mouse embryonic fibroblast) cells labeling PI 3 Kinase p85 alpha with purified ab225720 at 1/250 (10 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Flow cytometry analysis of PC-12 (rat adrenal gland pheochromocytoma) labeling PI 3 Kinase p85 alpha with purified ab225720 at 1/2500 dilution (1.03 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Flow cytometry analysis of NIH/3T3 (mouse embryo) labeling PI 3 Kinase p85 alpha with purified ab225720 at 1/250 dilution (10.32 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Flow cytometric analysis of MCF7 (human breast adenocarcinoma cell line) cell line labeling PI 3 Kinase p85 alpha with ab225720 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human melanoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human testis tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human breast adenocarcinoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human placenta tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab236440? Please let us know so that we can cite the reference in this datasheet.

ab236440 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com