Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651)

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Western blot - Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651)
  • Western blot - Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651)
  • Western blot - Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651)

Key features and details

  • Rabbit polyclonal to PI 3 Kinase p85 alpha (phospho Y607)
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

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Anti-PI 3 Kinase p85 alpha antibody [EPR18702] (ab191606)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody
    See all PI 3 Kinase p85 alpha primary antibodies

  • Description

    Rabbit polyclonal to PI 3 Kinase p85 alpha (phospho Y607)

  • Host species

    Rabbit

  • Specificity

    ab182651 detects endogenous level of PI 3 Kinase p85 only when phosphorylated at Tyrosine 607. 

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to Human PI 3 Kinase p85 alpha (phospho Y607) conjugated to keyhole limpet haemocyanin. Peptide sequence around phosphorylation site of Tyrosine 607 (D-Q-Y(p)-S-L) derived from Human PI3K p85.
    Database link: P27986

  • Positive control

    • WB: Insulin treated HeLa cell lysates. HeLa cells. Rat liver extract. MCF-7 cell lysate; mouse brain tissue lysate; rat brain tissue lysate. IHC-P: Human brain tissue.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab182651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration.
WB (1)
1/500 - 1/1000. Predicted molecular weight: 84 kDa.

Block membrane by incubating 1 hour at room temperature or overnight at 4 °C with shaking in Blocking Solution(5% nonfat milk, 1X TBS, 0.1% Tween-20). Incubate the membrane with diluted primary antibody for 2 hours at 37 °C, or overnight at 4 °C with agitation.

Samples require induction for efficent signal. Please refer to positive control section. 
Notes
IHC-P
Use at an assay dependent concentration.
WB
1/500 - 1/1000. Predicted molecular weight: 84 kDa.

Block membrane by incubating 1 hour at room temperature or overnight at 4 °C with shaking in Blocking Solution(5% nonfat milk, 1X TBS, 0.1% Tween-20). Incubate the membrane with diluted primary antibody for 2 hours at 37 °C, or overnight at 4 °C with agitation.

Samples require induction for efficent signal. Please refer to positive control section. 

Target

  • Function

    Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

  • Tissue specificity

    Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).

  • Sequence similarities

    Belongs to the PI3K p85 subunit family.
    Contains 1 Rho-GAP domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.

  • Domain

    The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.

  • Post-translational
    modifications

    Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
    Phosphorylated. Dephosphorylated by PTPRJ.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • GRB1 antibody
    • p85 alpha antibody
    • p85 antibody
    • P85A_HUMAN antibody
    • Phosphatidylinositol 3 kinase associated p 85 alpha antibody
    • Phosphatidylinositol 3 kinase regulatory 1 antibody
    • Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 1 (p85 alpha) antibody
    • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody
    • Phosphatidylinositol 3-kinase regulatory subunit alpha antibody
    • Phosphoinositide 3 kinase, regulatory subunit 1 (alpha) antibody
    • PI3 kinase p85 antibody
    • PI3 kinase p85 subunit alpha antibody
    • PI3-kinase regulatory subunit alpha antibody
    • PI3-kinase subunit p85-alpha antibody
    • PI3K antibody
    • PI3K p85 antibody
    • PI3K regulatory subunit alpha antibody
    • Pik3r1 antibody
    • PtdIns 3 kinase p85 alpha antibody
    • PtdIns-3-kinase regulatory subunit alpha antibody
    • PtdIns-3-kinase regulatory subunit p85-alpha antibody
    see all

Images

  • Western blot - Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651)
    Western blot - Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651)
    All lanes : Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651) at 1/500 dilution

    Lane 1 : Insulin treated HeLa cell lysates
    Lane 2 : HeLa cell lysates

    Secondary
    All lanes : HRP-conjugate secondary antibody

    Predicted band size: 84 kDa



    Blocking solution: 5% non-fat milk

    Blocking for 1 hour at room temperature

    Primary incubation overnight at 4°C

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651)

    Immunohistochemistry of human brain tissue labeling PI 3 Kinase p85 (phospho Y607) with ab182651 at 1/200.

  • Western blot - Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651)
    Western blot - Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651)
    All lanes : Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651) at 1/500 dilution

    Lane 1 : Rat liver extract
    Lane 2 : Rat liver extract with antigen-specific peptide

    Predicted band size: 84 kDa

  • Western blot - Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651)
    Western blot - Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651)
    All lanes : Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651) at 1/750 dilution

    Lane 1 : MCF-7 cell lysate with N-Peptide
    Lane 2 : MCF-7 cell lysate
    Lane 3 : MCF-7 cell lysate with P-Peptide
    Lane 4 : Mouse brain tissue lysate with N-Peptide
    Lane 5 : Mouse brain tissue lysate
    Lane 6 : Mouse brain tissue lysate with P-Peptide
    Lane 7 : Rat brain tissue lysate with N-Peptide
    Lane 8 : Rat brain tissue lysate
    Lane 9 : Rat brain tissue lysate with P-Peptide

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 84 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes

References (297)

Publishing research using ab182651? Please let us know so that we can cite the reference in this datasheet.

ab182651 has been referenced in 297 publications.

  • Yan J  et al. Integrative transcriptomic and proteomic analysis reveals CD9/ITGA4/PI3K-Akt axis mediates trabecular meshwork cell apoptosis in human glaucoma. J Cell Mol Med 24:814-829 (2020). PubMed: 31680442
  • Han Y  et al. Long non-coding RNA GAS5 regulates myocardial ischemia-reperfusion injury through the PI3K/AKT apoptosis pathway by sponging miR-532-5p. Int J Mol Med 45:858-872 (2020). PubMed: 31985016
  • Wang D  et al. Propofol Inhibits the Migration and Invasion of Glioma Cells by Blocking the PI3K/AKT Pathway Through miR-206/ROCK1 Axis. Onco Targets Ther 13:361-370 (2020). PubMed: 32021281
  • Wu HB  et al. Avß3 Single-Stranded DNA Aptamer Attenuates Vascular Smooth Muscle Cell Proliferation and Migration via Ras-PI3K/MAPK Pathway. Cardiovasc Ther 2020:6869856 (2020). PubMed: 32042311
  • Fu W  et al. Effect of miR-144-5p on the proliferation, migration, invasion and apoptosis of human umbilical vein endothelial cells by targeting RICTOR and its related mechanisms. Exp Ther Med 19:1817-1823 (2020). PubMed: 32104237
View all Publications for this product

Customer reviews and Q&As

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Western blot abreview for Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Guinea pig Tissue lysate - whole (Placenta)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-15%)
Loading amount
15 µg
Specification
Placenta
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Oct 20 2020

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