Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651)

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Overview

  • Product name

    Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody
    See all PI 3 Kinase p85 alpha primary antibodies

  • Description

    Rabbit polyclonal to PI 3 Kinase p85 alpha (phospho Y607)

  • Host species

    Rabbit

  • Specificity

    ab182651 detects endogenous level of PI 3 Kinase p85 only when phosphorylated at Tyrosine 607.

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to Human PI 3 Kinase p85 alpha (phospho Y607) conjugated to keyhole limpet haemocyanin. Peptide sequence around phosphorylation site of Tyrosine 607 (D-Q-Y(p)-S-L) derived from Human PI3K p85.
    Database link: P27986

  • Positive control

    • WB: Insulin treated HeLa cell lysates. HeLa cells treated with 20 µM hydrogen peroxide for 3 minutes.

Properties

Applications

Our Abpromise guarantee covers the use of ab182651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 84 kDa.

Block membrane by incubating 1 hour at room temperature or overnight at 4 °C with shaking in Blocking Solution(5% nonfat milk, 1X TBS, 0.1% Tween-20).

Incubate the membrane with diluted primary antibody for 2 hours at 37 °C, or overnight at 4 °C with agitation.

Target

  • Function

    Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

  • Tissue specificity

    Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).

  • Sequence similarities

    Belongs to the PI3K p85 subunit family.
    Contains 1 Rho-GAP domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.

  • Domain

    The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.

  • Post-translational
    modifications

    Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
    Phosphorylated. Dephosphorylated by PTPRJ.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • GRB1 antibody
    • p85 alpha antibody
    • p85 antibody
    • P85A_HUMAN antibody
    • Phosphatidylinositol 3 kinase associated p 85 alpha antibody
    • Phosphatidylinositol 3 kinase regulatory 1 antibody
    • Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 1 (p85 alpha) antibody
    • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody
    • Phosphatidylinositol 3-kinase regulatory subunit alpha antibody
    • Phosphoinositide 3 kinase, regulatory subunit 1 (alpha) antibody
    • PI3 kinase p85 antibody
    • PI3 kinase p85 subunit alpha antibody
    • PI3-kinase regulatory subunit alpha antibody
    • PI3-kinase subunit p85-alpha antibody
    • PI3K antibody
    • PI3K p85 antibody
    • PI3K regulatory subunit alpha antibody
    • Pik3r1 antibody
    • PtdIns 3 kinase p85 alpha antibody
    • PtdIns-3-kinase regulatory subunit alpha antibody
    • PtdIns-3-kinase regulatory subunit p85-alpha antibody
    see all

Images

  • Western blot - Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651)
    Western blot - Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651)
    All lanes : Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651) at 1/500 dilution

    Lane 1 : Insulin treated HeLa cell lysates
    Lane 2 : HeLa cell lysates

    Secondary
    All lanes : HRP-conjugate secondary antibody

    Predicted band size: 84 kDa



    Blocking solution: 5% non-fat milk

    Blocking for 1 hour at room temperature

    Primary incubation overnight at 4°C

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651)

    Immunohistochemistry of human brain tissue labeling PI 3 Kinase p85 (phospho Y607) with ab182651 at 1/200.

  • Western blot - Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651)
    Western blot - Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651)
    All lanes : Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651) at 1/500 dilution

    Lane 1 : Rat liver extract
    Lane 2 : Rat liver extract with antigen-specific peptide

    Predicted band size: 84 kDa

  • Western blot - Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651)
    Western blot - Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651)
    All lanes : Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651) at 1/750 dilution

    Lane 1 : MCF-7 cell lysate with N-Peptide
    Lane 2 : MCF-7 cell lysate
    Lane 3 : MCF-7 cell lysate with P-Peptide
    Lane 4 : Mouse brain tissue lysate with N-Peptide
    Lane 5 : Mouse brain tissue lysate
    Lane 6 : Mouse brain tissue lysate with P-Peptide
    Lane 7 : Rat brain tissue lysate with N-Peptide
    Lane 8 : Rat brain tissue lysate
    Lane 9 : Rat brain tissue lysate with P-Peptide

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 84 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes

References

Publishing research using ab182651? Please let us know so that we can cite the reference in this datasheet.

ab182651 has been referenced in 168 publications.

  • Yuan Y & Zheng Z Geniposide protects PC-12 cells against oxygen and glucose deprivation-induced injury by up-regulation of long-noncoding RNA H19. Life Sci 216:176-182 (2019). PubMed: 30472296
  • Cui LH  et al. Saikosaponin d ameliorates pancreatic fibrosis by inhibiting autophagy of pancreatic stellate cells via PI3K/Akt/mTOR pathway. Chem Biol Interact 300:18-26 (2019). PubMed: 30611790
  • Dong H  et al. Activation of LncRNA TINCR by H3K27 acetylation promotes Trastuzumab resistance and epithelial-mesenchymal transition by targeting MicroRNA-125b in breast Cancer. Mol Cancer 18:3 (2019). PubMed: 30621694
  • Xing Y & Li L Gastrodin protects rat cardiomyocytes H9c2 from hypoxia-induced injury by up-regulation of microRNA-21. Int J Biochem Cell Biol 109:8-16 (2019). PubMed: 30684569
  • Xin M  et al. Chinese herbal decoction of Wenshen Yangxue formula improved fertility and pregnancy rate in mice through PI3K/Akt signaling. J Cell Biochem 120:3082-3090 (2019). PubMed: 30474873
View all Publications for this product

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