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Signal Transduction Signaling Pathway Lipid Signaling Lipid Kinases
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Anti-PI 3 Kinase p85 beta antibody (ab131067)

  • Datasheet
  • SDS
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Western blot - Anti-PI 3 Kinase p85 beta antibody (ab131067)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 beta antibody (ab131067)
  • Western blot - Anti-PI 3 Kinase p85 beta antibody (ab131067)

Key features and details

  • Rabbit polyclonal to PI 3 Kinase p85 beta
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Recombinant human PI3K (p110 delta/p85 alpha) protein (Active) (ab125633)
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Overview

  • Product name

    Anti-PI 3 Kinase p85 beta antibody
    See all PI 3 Kinase p85 beta primary antibodies
  • Description

    Rabbit polyclonal to PI 3 Kinase p85 beta
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Rat
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human PI 3 Kinase p85 beta aa 447-461 (internal sequence).
    Sequence:

    KVYHQQYQDKSREYD

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Rat testis tissue lysate and HeLa and 293T whole cell lysates. IHC-P: Human mammary cancer tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 2.5% BSA, 0.1% Dibasic monohydrogen sodium phosphate, 0.45% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • Lipid Kinases
    • Immunology
    • Innate Immunity
    • TLR Signaling

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human PI 3 Kinase p85 beta protein (ab125568)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab131067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Mouse
Rat
Application Abreviews Notes
WB
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (PubMed:23604317). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement.
  • Involvement in disease

    Megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 1
  • Sequence similarities

    Belongs to the PI3K p85 subunit family.
    Contains 1 Rho-GAP domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • Domain

    The SH2 2 domain is required for interaction with FBXL2 and PTPN13.
  • Post-translational
    modifications

    Phosphorylated in response to signaling from activated receptor-type protein kinases (PubMed:19690332, PubMed:20068231). Dephosphorylated by PTPRJ (PubMed:18348712). Dephosphorylated at Tyr-655 by PTPN13. Phosphorylation of Tyr-655 impairs while its dephosphorylation promotes interaction with FBXL2 and SCF(FBXL2)-mediated polyubiquitination (PubMed:23604317).
    Ubiquitinated. Polyubiquitination by the SCF(FBXL2) complex probably promotes proteasomal degradation of PIK3R2.
  • Target information above from: UniProt accession O00459 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5296 Human
    • Entrez Gene: 18709 Mouse
    • Entrez Gene: 29741 Rat
    • Omim: 603157 Human
    • SwissProt: O00459 Human
    • SwissProt: O08908 Mouse
    • SwissProt: Q63788 Rat
    • Unigene: 371344 Human
    • Unigene: 12945 Mouse
    • Unigene: 22497 Rat
    see all
  • Alternative names

    • p85 antibody
    • p85 beta antibody
    • P85B antibody
    • P85B_HUMAN antibody
    • Phosphatidylinositol 3 kinase antibody
    • Phosphatidylinositol 3 kinase regulatory beta subunit antibody
    • Phosphatidylinositol 3 kinase regulatory subunit beta antibody
    • Phosphatidylinositol 3 kinase regulatory subunit polypeptide 2 antibody
    • Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 2 (p85 beta) antibody
    • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta antibody
    • Phosphatidylinositol 3-kinase regulatory subunit beta antibody
    • Phosphoinositide 3 kinase regulatory subunit 2 (beta) antibody
    • Phosphoinositide 3 kinase regulatory subunit 2 antibody
    • Phosphoinositide 3 kinase regulatory subunit polypeptide 2 (p85 beta) antibody
    • Phosphoinositide 3 kinase regulatory subunit polypeptide 2 antibody
    • Phosphoinositide 3 kinase, regulatory subunit 2 (beta) antibody
    • Phosphoinositide 3 kinase, regulatory subunit 2 (p85 beta) antibody
    • PI3 kinase p85 beta subunit antibody
    • PI3 kinase p85 subunit beta antibody
    • PI3-kinase regulatory subunit beta antibody
    • PI3-kinase subunit p85-beta antibody
    • PI3K antibody
    • PI3K regulatory subunit beta antibody
    • PIK3R 2 antibody
    • PIK3R2 antibody
    • PtdIns 3 kinase p85 beta antibody
    • PtdIns-3-kinase regulatory subunit beta antibody
    • PtdIns-3-kinase regulatory subunit p85-beta antibody
    see all

Images

  • Western blot - Anti-PI 3 Kinase p85 beta antibody (ab131067)
    Western blot - Anti-PI 3 Kinase p85 beta antibody (ab131067)
    All lanes : Anti-PI 3 Kinase p85 beta antibody (ab131067) at 0.5 µg/ml

    Lane 1 : Rat testis tissue lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 40 µg
    Lane 3 : HeLa whole cell lysate at 40 µg

    Predicted band size: 81 kDa
    Observed band size: 81 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 beta antibody (ab131067)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 beta antibody (ab131067)
    Immunohistochemical analysis of paraffin embedded Human Mammary Cancer Tissue labelled with ab131067 PI 3 Kinase p85 beta antibody at 1 µg/ml.
  • Western blot - Anti-PI 3 Kinase p85 beta antibody (ab131067)
    Western blot - Anti-PI 3 Kinase p85 beta antibody (ab131067)
    All lanes : Anti-PI 3 Kinase p85 beta antibody (ab131067) at 0.5 µg/ml

    Lane 1 : mouse liver tissue lysates
    Lane 2 : mouse brain tissue lysates

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG-HRP at 1/10000 dilution

    Predicted band size: 81 kDa

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (3)

    Publishing research using ab131067? Please let us know so that we can cite the reference in this datasheet.

    ab131067 has been referenced in 3 publications.

    • Jia Z  et al. miR-126 suppresses epithelial-to-mesenchymal transition and metastasis by targeting PI3K/AKT/Snail signaling of lung cancer cells. Oncol Lett 15:7369-7375 (2018). PubMed: 29725450
    • Song L  et al. MicroRNA-126 inhibits proliferation and metastasis by targeting pik3r2 in prostate cancer. Mol Med Rep 13:1204-10 (2016). PubMed: 26677064
    • Cao WJ  et al. Therapeutic Angiogenesis by Ultrasound-Mediated MicroRNA-126-3p Delivery. Arterioscler Thromb Vasc Biol 35:2401-11 (2015). PubMed: 26381870

    Customer reviews and Q&As

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