Anti-PI 3 Kinase p85 beta antibody (ab131067)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Anti-PI 3 Kinase p85 beta antibody
See all PI 3 Kinase p85 beta primary antibodies -
Description
Rabbit polyclonal to PI 3 Kinase p85 beta -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human PI 3 Kinase p85 beta aa 447-461 (internal sequence).
Sequence:KVYHQQYQDKSREYD
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Positive control
- WB: Rat testis tissue lysate and HeLa and 293T whole cell lysates. IHC-P: Human mammary cancer tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.1% Dibasic monohydrogen sodium phosphate, 0.45% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab131067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa). | |
| IHC-P | Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (PubMed:23604317). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement. -
Involvement in disease
Megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 1 -
Sequence similarities
Belongs to the PI3K p85 subunit family.
Contains 1 Rho-GAP domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
Domain
The SH2 2 domain is required for interaction with FBXL2 and PTPN13. -
Post-translational
modificationsPhosphorylated in response to signaling from activated receptor-type protein kinases (PubMed:19690332, PubMed:20068231). Dephosphorylated by PTPRJ (PubMed:18348712). Dephosphorylated at Tyr-655 by PTPN13. Phosphorylation of Tyr-655 impairs while its dephosphorylation promotes interaction with FBXL2 and SCF(FBXL2)-mediated polyubiquitination (PubMed:23604317).
Ubiquitinated. Polyubiquitination by the SCF(FBXL2) complex probably promotes proteasomal degradation of PIK3R2. - Information by UniProt
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Database links
- Entrez Gene: 5296 Human
- Entrez Gene: 18709 Mouse
- Entrez Gene: 29741 Rat
- Omim: 603157 Human
- SwissProt: O00459 Human
- SwissProt: O08908 Mouse
- SwissProt: Q63788 Rat
- Unigene: 371344 Human
see all -
Alternative names
- p85 antibody
- p85 beta antibody
- P85B antibody
see all
Images
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Western blot - Anti-PI 3 Kinase p85 beta antibody (ab131067)All lanes : Anti-PI 3 Kinase p85 beta antibody (ab131067) at 0.5 µg/ml
Lane 1 : Rat testis tissue lysate at 50 µg
Lane 2 : 293T whole cell lysate at 40 µg
Lane 3 : HeLa whole cell lysate at 40 µg
Predicted band size: 81 kDa
Observed band size: 81 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 beta antibody (ab131067)Immunohistochemical analysis of paraffin embedded Human Mammary Cancer Tissue labelled with ab131067 PI 3 Kinase p85 beta antibody at 1 µg/ml. -
Western blot - Anti-PI 3 Kinase p85 beta antibody (ab131067)All lanes : Anti-PI 3 Kinase p85 beta antibody (ab131067) at 0.5 µg/ml
Lane 1 : mouse liver tissue lysates
Lane 2 : mouse brain tissue lysates
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG-HRP at 1/10000 dilution
Predicted band size: 81 kDa
References
This product has been referenced in:
- Jia Z et al. miR-126 suppresses epithelial-to-mesenchymal transition and metastasis by targeting PI3K/AKT/Snail signaling of lung cancer cells. Oncol Lett 15:7369-7375 (2018). Read more (PubMed: 29725450) »
- Song L et al. MicroRNA-126 inhibits proliferation and metastasis by targeting pik3r2 in prostate cancer. Mol Med Rep 13:1204-10 (2016). Read more (PubMed: 26677064) »
