Anti-PI 3 Kinase p85 beta antibody (ab131067)
Key features and details
- Rabbit polyclonal to PI 3 Kinase p85 beta
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PI 3 Kinase p85 beta antibody
See all PI 3 Kinase p85 beta primary antibodies -
Description
Rabbit polyclonal to PI 3 Kinase p85 beta -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB MouseRat
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Immunogen
Synthetic peptide corresponding to Human PI 3 Kinase p85 beta aa 447-461 (internal sequence).
Sequence:KVYHQQYQDKSREYD
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Positive control
- WB: Rat testis tissue lysate and HeLa and 293T whole cell lysates. IHC-P: Human mammary cancer tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.1% Dibasic monohydrogen sodium phosphate, 0.45% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
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| WB |
Mouse
Rat
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| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
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| IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Notes |
|---|
|
WB
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa). |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (PubMed:23604317). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement. -
Involvement in disease
Megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 1 -
Sequence similarities
Belongs to the PI3K p85 subunit family.
Contains 1 Rho-GAP domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
Domain
The SH2 2 domain is required for interaction with FBXL2 and PTPN13. -
Post-translational
modificationsPhosphorylated in response to signaling from activated receptor-type protein kinases (PubMed:19690332, PubMed:20068231). Dephosphorylated by PTPRJ (PubMed:18348712). Dephosphorylated at Tyr-655 by PTPN13. Phosphorylation of Tyr-655 impairs while its dephosphorylation promotes interaction with FBXL2 and SCF(FBXL2)-mediated polyubiquitination (PubMed:23604317).
Ubiquitinated. Polyubiquitination by the SCF(FBXL2) complex probably promotes proteasomal degradation of PIK3R2. - Information by UniProt
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Database links
- Entrez Gene: 5296 Human
- Entrez Gene: 18709 Mouse
- Entrez Gene: 29741 Rat
- Omim: 603157 Human
- SwissProt: O00459 Human
- SwissProt: O08908 Mouse
- SwissProt: Q63788 Rat
- Unigene: 371344 Human
see all -
Alternative names
- p85 antibody
- p85 beta antibody
- P85B antibody
see all
Images
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Western blot - Anti-PI 3 Kinase p85 beta antibody (ab131067)All lanes : Anti-PI 3 Kinase p85 beta antibody (ab131067) at 0.5 µg/ml
Lane 1 : Rat testis tissue lysate at 50 µg
Lane 2 : 293T whole cell lysate at 40 µg
Lane 3 : HeLa whole cell lysate at 40 µg
Predicted band size: 81 kDa
Observed band size: 81 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 beta antibody (ab131067)Immunohistochemical analysis of paraffin embedded Human Mammary Cancer Tissue labelled with ab131067 PI 3 Kinase p85 beta antibody at 1 µg/ml. -
Western blot - Anti-PI 3 Kinase p85 beta antibody (ab131067)All lanes : Anti-PI 3 Kinase p85 beta antibody (ab131067) at 0.5 µg/ml
Lane 1 : mouse liver tissue lysates
Lane 2 : mouse brain tissue lysates
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG-HRP at 1/10000 dilution
Predicted band size: 81 kDa
Protocols
References (3)
ab131067 has been referenced in 3 publications.
- Jia Z et al. miR-126 suppresses epithelial-to-mesenchymal transition and metastasis by targeting PI3K/AKT/Snail signaling of lung cancer cells. Oncol Lett 15:7369-7375 (2018). PubMed: 29725450
- Song L et al. MicroRNA-126 inhibits proliferation and metastasis by targeting pik3r2 in prostate cancer. Mol Med Rep 13:1204-10 (2016). PubMed: 26677064
- Cao WJ et al. Therapeutic Angiogenesis by Ultrasound-Mediated MicroRNA-126-3p Delivery. Arterioscler Thromb Vasc Biol 35:2401-11 (2015). PubMed: 26381870
