Anti-PI 3 Kinase p85 beta antibody [T15] (ab28356)
- Datasheet
- References (6)
- Protocols
Overview
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Product nameAnti-PI 3 Kinase p85 beta antibody [T15]
See all PI 3 Kinase p85 beta primary antibodies -
DescriptionMouse monoclonal [T15] to PI 3 Kinase p85 beta
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Host speciesMouse
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SpecificityT15 recognises the p85 beta subunit of bovine PI 3 kinase. It does not cross react with the p85 alpha subunit.
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Tested applicationsSuitable for: IHC-Fr, IP, WBmore details
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Species reactivityReacts with: Human, Monkey
Predicted to work with: Mouse, Rat
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Immunogen
Balculovirus expressed (Recombinant) bovine P85 beta subunit of PI 3 kinase.
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Positive control
- Lysates of SF9 cells infected with baculovirus expressing p85 beta.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: 0.09% Sodium azide
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Concentration information loading... -
PurityProtein G purified
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Purification notesThis antibody is purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
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ClonalityMonoclonal
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Clone numberT15
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MyelomaNS1
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab28356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| IHC-Fr | Use at an assay dependent concentration. | |
| IP | 1/25. Use 25ul. | |
| WB | 1/1000 - 1/5000. Predicted molecular weight: 82 kDa. |
Target
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FunctionRegulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (PubMed:23604317). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement.
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Involvement in diseaseMegalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 1
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Sequence similaritiesBelongs to the PI3K p85 subunit family.
Contains 1 Rho-GAP domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
DomainThe SH2 2 domain is required for interaction with FBXL2 and PTPN13.
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Post-translational
modificationsPhosphorylated in response to signaling from activated receptor-type protein kinases (PubMed:19690332, PubMed:20068231). Dephosphorylated by PTPRJ (PubMed:18348712). Dephosphorylated at Tyr-655 by PTPN13. Phosphorylation of Tyr-655 impairs while its dephosphorylation promotes interaction with FBXL2 and SCF(FBXL2)-mediated polyubiquitination (PubMed:23604317).
Ubiquitinated. Polyubiquitination by the SCF(FBXL2) complex probably promotes proteasomal degradation of PIK3R2. - Information by UniProt
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Database links
- Entrez Gene: 5296 Human
- Entrez Gene: 18709 Mouse
- Entrez Gene: 29741 Rat
- Omim: 603157 Human
- SwissProt: O00459 Human
- SwissProt: O08908 Mouse
- SwissProt: Q63788 Rat
- Unigene: 371344 Human
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Alternative names
- p85 antibody
- p85 beta antibody
- P85B antibody
see all
Images
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![Western blot - Anti-PI 3 Kinase p85 beta antibody [T15] (ab28356)](https://www.abcam.com/ps/datasheet/images/28/ab28356/PI-3-Kinase-p85-beta-Primary-antibodies-ab28356-1.jpg)
Western blot - Anti-PI 3 Kinase p85 beta antibody [T15] (ab28356)Western Blotting of HEK293 and HepG2 cell lysates with Mouse anti Bovine PI-3 kinase p85 subunit beta (ab28356), recombinant p85ß is included as a positive control.
Datasheets and documents
References
This product has been referenced in:
- Kuchay S et al. PTEN counteracts FBXL2 to promote IP3R3- and Ca2+-mediated apoptosis limiting tumour growth. Nature 546:554-558 (2017). Read more (PubMed: 28614300) »
- Felli N et al. AP2a controls the dynamic balance between miR-126&126* and miR-221&222 during melanoma progression. Oncogene 35:3016-26 (2016). WB . Read more (PubMed: 26434590) »
