Anti-PI 3 Kinase p85 beta antibody [T15] (ab28356)
![Western blot - Anti-PI 3 Kinase p85 beta antibody [T15] (ab28356)](https://www.abcam.com/ps/products/28/ab28356/Images/ab28356-409564-anti-pi-3-kinase-p85-beta-antibody-t15-western-blot-hek-293t-lysates.jpg "Western blot - Anti-PI 3 Kinase p85 beta antibody [T15] (ab28356)")
Key features and details
- Mouse monoclonal [T15] to PI 3 Kinase p85 beta
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-PI 3 Kinase p85 beta antibody [T15]
See all PI 3 Kinase p85 beta primary antibodies -
Description
Mouse monoclonal [T15] to PI 3 Kinase p85 beta -
Host species
Mouse -
Specificity
T15 recognises the p85 beta subunit of bovine PI 3 kinase. It does not cross react with the p85 alpha subunit. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Balculovirus expressed (Recombinant) bovine P85 beta subunit of PI 3 kinase.
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Positive control
- WB: HEK293T cell lysate; Lysates of SF9 cells infected with baculovirus expressing p85 beta.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.09% Sodium azide -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
This antibody is purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
T15 -
Myeloma
NS1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 82 kDa.
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| Notes |
|---|
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WB
1/1000 - 1/5000. Predicted molecular weight: 82 kDa. |
Target
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Function
Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (PubMed:23604317). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement. -
Involvement in disease
Megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 1 -
Sequence similarities
Belongs to the PI3K p85 subunit family.
Contains 1 Rho-GAP domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
Domain
The SH2 2 domain is required for interaction with FBXL2 and PTPN13. -
Post-translational
modificationsPhosphorylated in response to signaling from activated receptor-type protein kinases (PubMed:19690332, PubMed:20068231). Dephosphorylated by PTPRJ (PubMed:18348712). Dephosphorylated at Tyr-655 by PTPN13. Phosphorylation of Tyr-655 impairs while its dephosphorylation promotes interaction with FBXL2 and SCF(FBXL2)-mediated polyubiquitination (PubMed:23604317).
Ubiquitinated. Polyubiquitination by the SCF(FBXL2) complex probably promotes proteasomal degradation of PIK3R2. - Information by UniProt
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Database links
- Entrez Gene: 5296 Human
- Entrez Gene: 18709 Mouse
- Entrez Gene: 29741 Rat
- Omim: 603157 Human
- SwissProt: O00459 Human
- SwissProt: O08908 Mouse
- SwissProt: Q63788 Rat
- Unigene: 371344 Human
see all -
Alternative names
- p85 antibody
- p85 beta antibody
- P85B antibody
see all
Images
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Western blot - Anti-PI 3 Kinase p85 beta antibody [T15] (ab28356)All lanes : Anti-PI 3 Kinase p85 beta antibody [T15] (ab28356) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : PIK3R2 knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 82 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?Lanes 1- 2: Merged signal (red and green). Green - ab28356 observed at 85 kDa. Red - Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) observed at 37 kDa.
ab28356 was shown to react with PI 3 Kinase p85 beta in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266799 (knockout cell lysate ab257586) was used. Wild-type HEK-293T and PIK3R2 knockout HEK-293T cell lysates were subjected to SDS-PAGE. ab28356 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye®800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye®680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-PI 3 Kinase p85 beta antibody [T15] (ab28356)](https://www.abcam.com/ps/products/28/ab28356/Images/ab28356-39274-anti-pi-3-kinase-p85-beta-antibody-t15-western-blot.jpg)
Western blot - Anti-PI 3 Kinase p85 beta antibody [T15] (ab28356)Western Blotting of HEK293 and HepG2 cell lysates with Mouse anti Bovine PI-3 kinase p85 subunit beta (ab28356), recombinant p85ß is included as a positive control.
Protocols
Datasheets and documents
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Datasheet download
References (12)
ab28356 has been referenced in 12 publications.
- Zheng D et al. Long Non-coding RNA Gas5 Is Associated With Preeclampsia and Regulates Biological Behaviors of Trophoblast via MicroRNA-21. Front Genet 11:188 (2020). PubMed: 32194641
- Pedini F et al. Joint action of miR-126 and MAPK/PI3K inhibitors against metastatic melanoma. Mol Oncol 13:1836-1854 (2019). PubMed: 31115969
- Yang MH et al. Reduction of aluminum ion neurotoxicity through a small peptide application - NAP treatment of Alzheimer's disease. J Food Drug Anal 27:551-564 (2019). PubMed: 30987727
- Dai K et al. Kbtbd2 inhibits the cytotoxic activity of immortalized NK cells through down-regulating mTOR signaling in a mouse hepatocellular carcinoma model. Eur J Immunol 48:683-695 (2018). PubMed: 29331106
- Kuchay S et al. PTEN counteracts FBXL2 to promote IP3R3- and Ca2+-mediated apoptosis limiting tumour growth. Nature 546:554-558 (2017). PubMed: 28614300
