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Rabbit monoclonal [EPR5303(2)] to PI 3 Kinase regulatory subunit 4
, Flow Cyt
, ICC/IFmore detailsUnsuitable for:
Mouse, Rat, Human
Synthetic peptide within Human PI 3 Kinase regulatory subunit 4 aa 1300 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q99570
(Peptide available as
- Raji cell lysate, Jurkat cell lysate, Molt-4 cell lysate, 293T cell lysate, C6 cell lysate and Raw 264.7 cell lysate.
Human kidney tissue.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Tissue culture supernatant
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 153 kDa).Can be blocked with PI 3 Kinase regulatory subunit 4 peptide (ab183696).
||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
||1/100 - 1/250.
Is unsuitable for IP.
Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 3 HEAT repeats.
Contains 1 protein kinase domain.
Contains 7 WD repeats.
Information by UniProt
- MGC102700 antibody
- P150 antibody
- Phosphatidylinositol 3 kinase associated p150 antibody
Western blot - Anti-PI 3 Kinase regulatory subunit 4 antibody [EPR5303(2)] (ab124817)
All lanes : Anti-PI 3 Kinase regulatory subunit 4 antibody [EPR5303(2)] (ab124817) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Molt-4 cell lysate
Lane 4 : 293T cell lysate
Lane 5 : C6 cell lysate
Lane 6 : Raw 264.7 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 153 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase regulatory subunit 4 antibody [EPR5303(2)] (ab124817)
ab124817 at 1/100 dilution, staining PI 3 Kinase regulatory subunit 4 in Formalin fixed Paraffin-embedded Human kidney tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"