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Immunology Immune Interventions Transplantation
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RecombinantRabMAb

Recombinant Anti-PI-9 antibody [EPR8186(2)] (ab150400)

  • Datasheet
  • SDS
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Western blot - Anti-PI-9 antibody [EPR8186(2)] (ab150400)
  • Anti-PI-9 antibody [EPR8186(2)] (ab150400)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8186(2)] to PI-9
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

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Recombinant Human PI-9 protein (ab127323)
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Overview

  • Product name

    Anti-PI-9 antibody [EPR8186(2)]
    See all PI-9 primary antibodies
  • Description

    Rabbit monoclonal [EPR8186(2)] to PI-9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PI-9 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Ramos and Human placenta lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8186(2)
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immune Interventions
    • Transplantation
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Protease inhibitors
    • Serine protease inhibitors
    • SERPINs

Associated products

  • Alternative Versions

    • Anti-PI-9 antibody [EPR8186(2)] - BSA and Azide free (ab248969)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Ramos whole cell lysate (ab3955)
  • Recombinant Protein

    • Recombinant Human PI-9 protein (ab127323)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab150400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Granzyme B inhibitor.
    • Sequence similarities

      Belongs to the serpin family. Ov-serpin subfamily.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession P50453 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5272 Human
      • Omim: 601799 Human
      • SwissProt: P50453 Human
      • Unigene: 104879 Human
      • Alternative names

        • CAP 3 antibody
        • CAP-3 antibody
        • CAP3 antibody
        • Cytoplasmic antiproteinase 3 antibody
        • Peptidase inhibitor 9 antibody
        • PI-9 antibody
        • PI9 antibody
        • Protease inhibitor 9 antibody
        • Protease inhibitor 9 (ovalbumin type) antibody
        • Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 9 antibody
        • Serpin B9 antibody
        • Serpin peptidase inhibitor clade B (ovalbumin) member 9 antibody
        • Serpin peptidase inhibitor clade B member 9 antibody
        • SERPINB9 antibody
        • SPB9_HUMAN antibody
        see all

      Images

      • Western blot - Anti-PI-9 antibody [EPR8186(2)] (ab150400)
        Western blot - Anti-PI-9 antibody [EPR8186(2)] (ab150400)
        All lanes : Anti-PI-9 antibody [EPR8186(2)] (ab150400) at 1/1000 dilution

        Lane 1 : Ramos lysate
        Lane 2 : Human placenta lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 42 kDa



        Note: Dilute primary antibody in 1% BSA.
      • Anti-PI-9 antibody [EPR8186(2)] (ab150400)
        Anti-PI-9 antibody [EPR8186(2)] (ab150400)

      Protocols

      • Western blot protocols
      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab150400? Please let us know so that we can cite the reference in this datasheet.

      ab150400 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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