Overview

  • Product name

    Anti-PI3 Kinase p110 beta antibody
    See all PI3 Kinase p110 beta primary antibodies
  • Description

    Rabbit polyclonal to PI3 Kinase p110 beta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment (His-tag) corresponding to Human PI3 Kinase p110 beta aa 321-507. (Expressed in E.coli).
    Sequence:

    RIISHVWENNNPFQIVLVKGNKLNTEETVKVHVRAGLFHGTELLCKTIVS SEVSGKNDHIWNEPLEFDINICDLPRMARLCFAVYAVLDKVKTKKSTKTI NPSKYQTIRKAGKVHYPVAWVNTMVFDFKGQLRTGDIILHSWSSFPDELE EMLNPMGTVQTNPYTENATALHVKFPENKKQPYYYPP


    Database link: P42338

  • Positive control

    • IHC-P: Human cerebrum, liver, kidney, breast cancer and prostate cancer tissues. WB: HeLa, HepG2, Jurkat and K562 cell lysates; Recombinant human PI3 Kinase p110 beta protein. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab232951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 - 20 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 123 kDa.
IHC-P Use a concentration of 5 - 20 µg/ml.

Target

  • Function

    Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
  • Tissue specificity

    Expressed ubiquitously.
  • Pathway

    Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
  • Sequence similarities

    Belongs to the PI3/PI4-kinase family.
    Contains 1 PI3K/PI4K domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5-bisphosphate 3-kinase 110 kDa catalytic subunit beta antibody
    • 5-bisphosphate 3-kinase catalytic subunit beta isoform antibody
    • DKFZp779K1237 antibody
    • MGC133043 antibody
    • OTTHUMP00000216901 antibody
    • OTTHUMP00000216904 antibody
    • p110 BETA antibody
    • p110Beta antibody
    • Phosphatidylinositol 3 kinase catalytic beta polypeptide antibody
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit beta antibody
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit beta isoform antibody
    • Phosphatidylinositol-4 antibody
    • Phosphoinositide 3 kinase catalytic beta polypeptide antibody
    • PI3 kinase p110 subunit beta antibody
    • PI3-kinase subunit beta antibody
    • PI3K antibody
    • PI3K beta antibody
    • PI3K-beta antibody
    • PI3Kbeta antibody
    • PI3KCB antibody
    • PIK3C1 antibody
    • Pik3cb antibody
    • PK3CB_HUMAN antibody
    • PtdIns 3 kinase p110 antibody
    • PtdIns-3-kinase subunit beta antibody
    • PtdIns-3-kinase subunit p110-beta antibody
    see all

Images

  • Anti-PI3 Kinase p110 beta antibody (ab232951) at 2 µg/ml + Recombinant human PI3 Kinase p110 beta protein

    Predicted band size: 123 kDa

  • Paraffin-embedded human cerebrum tissue stained for PI3 Kinase p110 beta antibody using ab232951 at 20 µg/ml in immunohistochemical analysis. DAB staining.

  • Paraffin-embedded human liver tissue stained for PI3 Kinase p110 beta antibody using ab232951 at 20 µg/ml in immunohistochemical analysis. DAB staining.

  • Paraffin-embedded human kidney tissue stained for PI3 Kinase p110 beta antibody using ab232951 at 20 µg/ml in immunohistochemical analysis. DAB staining.

  • Paraffin-embedded human breast cancer tissue stained for PI3 Kinase p110 beta antibody using ab232951 at 20 µg/ml in immunohistochemical analysis. DAB staining.

  • Paraffin-embedded human prostate cancer tissue stained for PI3 Kinase p110 beta antibody using ab232951 at 20 µg/ml in immunohistochemical analysis. DAB staining.

  • All lanes : Anti-PI3 Kinase p110 beta antibody (ab232951) at 2 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 4 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate

    Secondary
    All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

    Predicted band size: 123 kDa

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling PI3 Kinase p110 beta using ab232951 at 20µg/mL in ICC/IF. FITC staining.

References

ab232951 has not yet been referenced specifically in any publications.

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