Key features and details
- Rabbit polyclonal to PI3 Kinase p110 beta
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
Product nameAnti-PI3 Kinase p110 beta antibody
See all PI3 Kinase p110 beta primary antibodies
DescriptionRabbit polyclonal to PI3 Kinase p110 beta
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human, Pig
Predicted to work with: Rat
Recombinant fragment (His-tag) corresponding to Human PI3 Kinase p110 beta aa 807-1070. (Expressed in E.coli).
GDDLRQDMLTLQMLRLMDLLWKEAGLDLRMLPYGCLATGDRSGLIEVVST SETIADIQLNSSNVAAAAAFNKDALLNWLKEYNSGDDLDRAIEEFTLSCA GYCVASYVLGIGDRHSDNIMVKKTGQLFHIDFGHILGNFKSKFGIKRERV PFILTYDFIHVIQQGKTGNTEKFGRFRQCCEDAYLILRRHGNLFITLFAL MLTAGLPELTSVKDIQYLKDSLALGKSEEEALKQFKQKFDEALRESWTTK VNWMAHTVRKDYRS
Database link: P42338
- WB: Pig cerebrum lysate; Mouse parotid gland lysate; Recombinant human PI3 Kinase p110 beta protein. IHC-P: Human prostate, liver cancer, liver, kidney and stomach tissue. ICC/IF: HepG2 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml.|
|ICC/IF||Use a concentration of 5 - 20 µg/ml.|
FunctionPhosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
Tissue specificityExpressed ubiquitously.
PathwayPhospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
Sequence similaritiesBelongs to the PI3/PI4-kinase family.
Contains 1 PI3K/PI4K domain.
- Information by UniProt
- 5-bisphosphate 3-kinase 110 kDa catalytic subunit beta antibody
- 5-bisphosphate 3-kinase catalytic subunit beta isoform antibody
- DKFZp779K1237 antibody
Anti-PI3 Kinase p110 beta antibody (ab232997) at 2 µg/ml + Mouse parotid gland lysate
HRP-Linked Guinea pig Anti-Rabbit at 1/1000 dilution
Formalin-fixed, paraffin-embedded human stomach tissue stained for PI3 Kinase p110 beta using ab232997 at 30 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human kidney tissue stained for PI3 Kinase p110 beta using ab232997 at 30 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human liver tissue stained for PI3 Kinase p110 beta using ab232997 at 30 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human liver cancer tissue stained for PI3 Kinase p110 beta using ab232997 at 30 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human prostate tissue stained for PI3 Kinase p110 beta using ab232997 at 30 µg/ml in immunohistochemical analysis. DAB staining.
HepG2 (human liver hepatocellular carcinoma cell line) stained for PI3 Kinase p110 beta (green) at 20 µg/mL in ICC/IF. FITC-labeled secondary antibody.
Anti-PI3 Kinase p110 beta antibody (ab232997) at 2 µg/ml + Pig cerebrum lysate
Anti-PI3 Kinase p110 beta antibody (ab232997) at 2 µg/ml + Recombinant human PI3 Kinase p110 beta protein
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232997 has been referenced in 3 publications.
- Wu YQ et al. PABPC1L depletion inhibits proliferation and migration via blockage of AKT pathway in human colorectal cancer cells. Oncol Lett 17:3439-3445 (2019). PubMed: 30867782
- Polo S et al. Treatment With the Delta Opioid Agonist UFP-512 Alleviates Chronic Inflammatory and Neuropathic Pain: Mechanisms Implicated. Front Pharmacol 10:283 (2019). PubMed: 30971925
- Díaz AF et al. Analgesic and Antidepressant Effects of Oltipraz on Neuropathic Pain in Mice by Modulating Microglial Activation. J Clin Med 8:N/A (2019). PubMed: 31234342