Overview

  • Product name

    Anti-PI3 Kinase p110 beta antibody
    See all PI3 Kinase p110 beta primary antibodies
  • Description

    Rabbit polyclonal to PI3 Kinase p110 beta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Monkey
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 509 and 790 of Human PI3 Kinase p110 beta (NP_006210)

  • Positive control

    • H1299 whole cell lysate; A431, HeLa, HepG2 and Raji cells

Properties

Applications

Our Abpromise guarantee covers the use of ab97322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 123 kDa.

Target

  • Function

    Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
  • Tissue specificity

    Expressed ubiquitously.
  • Pathway

    Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
  • Sequence similarities

    Belongs to the PI3/PI4-kinase family.
    Contains 1 PI3K/PI4K domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5-bisphosphate 3-kinase 110 kDa catalytic subunit beta antibody
    • 5-bisphosphate 3-kinase catalytic subunit beta isoform antibody
    • DKFZp779K1237 antibody
    • MGC133043 antibody
    • OTTHUMP00000216901 antibody
    • OTTHUMP00000216904 antibody
    • p110 BETA antibody
    • p110Beta antibody
    • Phosphatidylinositol 3 kinase catalytic beta polypeptide antibody
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit beta antibody
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit beta isoform antibody
    • Phosphatidylinositol-4 antibody
    • Phosphoinositide 3 kinase catalytic beta polypeptide antibody
    • PI3 kinase p110 subunit beta antibody
    • PI3-kinase subunit beta antibody
    • PI3K antibody
    • PI3K beta antibody
    • PI3K-beta antibody
    • PI3Kbeta antibody
    • PI3KCB antibody
    • PIK3C1 antibody
    • Pik3cb antibody
    • PK3CB_HUMAN antibody
    • PtdIns 3 kinase p110 antibody
    • PtdIns-3-kinase subunit beta antibody
    • PtdIns-3-kinase subunit p110-beta antibody
    see all

Images

  • Anti-PI3 Kinase p110 beta antibody (ab97322) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 123 kDa



    7.5% SDS PAGE

References

ab97322 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question

Hello,

We have a quality complaint for item ab55593. Please check attached file for detail.

We request money back.

Thanks,


Item number: ab55593

Order number: 1049169



General Information

Antibody storage conditions?





-20









Description of the problem (high background, wrong band size, more bands, no band etc.)





no band









Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)





Human/Cell extract









Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)





RIPA buffer containing protease and phosphatase inhibitor cocktails (Roche Diagnostics) and also heating sample







Amount of protein loaded





60, 80μg of total protein extracts









Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)





10% SDS-PAGE gels









Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)





nitrocellulose membranes (Milliopore) 90 min

The membranes were blocked with 5% milk powder, washed with TBST







Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)





Primeary antibody purchased from Abcom named ab55593

Primary antibody is diluted with 4% BSA. And the membranes were incubated overnight at 4℃. The membranes were washed with TBST three times.







Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)





peroxidase-conjugated anti-mouse IgG (Sigma) as a secondary antibody

Incubation for 2 hours was at room temperature.

Also washed three times by TBST.







Detection method (ECL, ECLPlus etc.)





Western Blotting luminal reagent (Santa cruz)







Positive and negative controls used (please specify)





Gapdh as positive control









Instruction

Repeat times: 3


Our western blotting experiment is based on the instruction provided by your company, but we got no band. At the same time, we used the protein of 293T cell (published by some paper) to repeat the experiment and we got the same result.

We have prolonged the time of the incubation and exposure, and also use the Concentration 1:200, but there is still no band of PI3KCB. There is no problem with our technology of western blotting, because we have got the target band of PI3KC3 by antibody from CST.

Finally, I hope that your company can make up our loss and solve the problems as soon as possible.

Read More
Answer

Thank you for your enquiry regarding ab55593 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guarantee period. This means that in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

As the datasheet indicates this antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

Would you be so kind to confirm the sample types used (species, cells, tissues)?

We have several alternative antibodies against the same target (PI3 Kinase p110 beta - ab32569, ab97322, ab1678) and I could offer you a free of charge replacement, or a credit note or a full refund as compensation.

Thank you for your understanding and co-operation in this matter. I look forward to hearing from you and hope to solve this problem as soon as possible.

Read More

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