Validated using a knockout cell line

Recombinant Anti-PI3 Kinase p110 beta antibody [Y384] (ab32569)


  • Product name

    Anti-PI3 Kinase p110 beta antibody [Y384]
    See all PI3 Kinase p110 beta primary antibodies
  • Description

    Rabbit monoclonal [Y384] to PI3 Kinase p110 beta
  • Host species

  • Specificity

    ab32569 recognises PI3-Kinases (PI3-Ks).
  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
    Unsuitable for: ICC or IHC
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide within Human PI3 Kinase p110 beta. The exact sequence is proprietary.

  • Positive control

    • Jurkat whole cell lysate (ab7899).
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab32569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 110 kDa (predicted molecular weight: 123 kDa).
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.
  • Application notes
    Is unsuitable for ICC or IHC.
  • Target

    • Function

      Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
    • Tissue specificity

      Expressed ubiquitously.
    • Pathway

      Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
    • Sequence similarities

      Belongs to the PI3/PI4-kinase family.
      Contains 1 PI3K/PI4K domain.
    • Information by UniProt
    • Database links

    • Alternative names

      • 5-bisphosphate 3-kinase 110 kDa catalytic subunit beta antibody
      • 5-bisphosphate 3-kinase catalytic subunit beta isoform antibody
      • DKFZp779K1237 antibody
      • MGC133043 antibody
      • OTTHUMP00000216901 antibody
      • OTTHUMP00000216904 antibody
      • p110 BETA antibody
      • p110Beta antibody
      • Phosphatidylinositol 3 kinase catalytic beta polypeptide antibody
      • Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit beta antibody
      • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit beta isoform antibody
      • Phosphatidylinositol-4 antibody
      • Phosphoinositide 3 kinase catalytic beta polypeptide antibody
      • PI3 kinase p110 subunit beta antibody
      • PI3-kinase subunit beta antibody
      • PI3K antibody
      • PI3K beta antibody
      • PI3K-beta antibody
      • PI3Kbeta antibody
      • PI3KCB antibody
      • PIK3C1 antibody
      • Pik3cb antibody
      • PK3CB_HUMAN antibody
      • PtdIns 3 kinase p110 antibody
      • PtdIns-3-kinase subunit beta antibody
      • PtdIns-3-kinase subunit p110-beta antibody
      see all


    • Lane 1: Wild-type HAP1 cell lysate (40 µg)
      Lane 2: PI3 Kinase p110 beta knockout HAP1 cell lysate (40 µg)
      Lane 3: Jurkat cell lysate (40 µg)
      Lane 4: K562 cell lysate (40 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab32569 observed at 125 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab32569 was shown to recognize PI3 Kinase p110 beta when PI3 Kinase p110 beta knockout samples were used, along with additional cross-reactive bands. Wild-type and PI3 Kinase p110 beta knockout samples were subjected to SDS-PAGE. Ab32569 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • Anti-PI3 Kinase p110 beta antibody [Y384] (ab32569) at 1/500 dilution + Jurkat cell lysate

      Predicted band size: 123 kDa
      Observed band size: 110 kDa
      why is the actual band size different from the predicted?


    This product has been referenced in:

    • Li P  et al. Expression and Comparison of Cbl-b in Lung Squamous Cell Carcinoma and Adenocarcinoma. Med Sci Monit 24:623-635 (2018). Read more (PubMed: 29384143) »
    • Yang Y  et al. Microarray pathway analysis indicated that mitogen-activated protein kinase/extracellular signal-regulated kinase and insulin growth factor 1 signaling pathways were inhibited by small interfering RNA against AT-rich interactive domain 1A in endometrial cancer. Oncol Lett 15:1829-1838 (2018). Read more (PubMed: 29399196) »
    See all 12 Publications for this product

    Customer reviews and Q&As

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    Western blot
    Human Cell lysate - whole cell (cancer cells)
    Loading amount
    15 µg
    cancer cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

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    Submitted Jun 07 2007

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