Product nameAnti-PI4 kinase beta/PI4KB antibody
See all PI4 kinase beta/PI4KB primary antibodies
DescriptionRabbit polyclonal to PI4 kinase beta/PI4KB
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Baboon, Rhesus monkey, Gorilla, Orangutan
- Whole cell lysate from HeLa, 293T and Jurkat cells. This antibody gave a positive result when used in the following methanol fixed cell lines: Panc-1
This product was previously labelled as PI4 kinase beta
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Note: pH 7.0 to 8.0
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab134756 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 91 kDa.|
|IP||Use 2-10 µg for 1000 µg of chromatin.|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionPhosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking.
Tissue specificityWidely expressed with highest levels in heart, skeletal muscle, pancreas, testis and ovary. Weakly expressed in liver.
Sequence similaritiesBelongs to the PI3/PI4-kinase family. Type III PI4K subfamily.
Contains 1 PI3K/PI4K domain.
Contains 1 PIK helical domain.
Cellular localizationEndomembrane system. Mitochondrion outer membrane. Rough endoplasmic reticulum membrane. Golgi apparatus. Cytoplasm, perinuclear region. Found in the outer membrane of mitochondria and membranes of the rough endoplasmic reticulum. Recruited to the Golgi complex by the small GTPase ARF to stimulate the synthesis of phosphatidylinositol 4,5-bisphosphate (PIP2) on the Golgi complex.
- Information by UniProt
- DKFZp686O1820 antibody
- FLJ30129 antibody
- NPIK antibody
All lanes : Anti-PI4 kinase beta/PI4KB antibody (ab134756) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from 293T cells at 50 µg
Lane 4 : Whole cell lysate from Jurkat cells at 50 µg
Developed using the ECL technique.
Predicted band size: 91 kDa
Exposure time: 10 seconds
Western Blot Gel 4-8%
ICC/IF image of ab134756 stained Panc-1 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab134756 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Lane 1: Detection of Human PI4 kinase beta/PI4KB by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab134756 at 6 µg/mg lysate for IP.
Lane 2: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized a downstream epitope of PI4 kinase beta/PI4KB.
Lane 3: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab134756 at 1 µg/ml.
Western Blot Gel 4-8%
Developed using the ECL technique with an exposure time of 3 seconds.
ab134756 has not yet been referenced specifically in any publications.