Overview

  • Product name

    Anti-PI4 kinase beta/PI4KB antibody
    See all PI4 kinase beta/PI4KB primary antibodies
  • Description

    Rabbit polyclonal to PI4 kinase beta/PI4KB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Baboon, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human PI4 kinase beta/PI4KB aa 1-50 (N terminal). (CAA09495.1)
    Sequence:

    MGDTVVEPAP LKPTSEPTSG PPGNNGGSLL SVITEGVGEL SVIDPEVAQK ACQEVLEKVK

  • Positive control

    • Whole cell lysate from HeLa, 293T and Jurkat cells. This antibody gave a positive result when used in the following methanol fixed cell lines: Panc-1
  • General notes

     This product was previously labelled as PI4 kinase beta

     

Properties

Applications

Our Abpromise guarantee covers the use of ab134756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 91 kDa.
IP Use 2-10 µg for 1000 µg of chromatin.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking.
  • Tissue specificity

    Widely expressed with highest levels in heart, skeletal muscle, pancreas, testis and ovary. Weakly expressed in liver.
  • Sequence similarities

    Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.
    Contains 1 PI3K/PI4K domain.
    Contains 1 PIK helical domain.
  • Cellular localization

    Endomembrane system. Mitochondrion outer membrane. Rough endoplasmic reticulum membrane. Golgi apparatus. Cytoplasm, perinuclear region. Found in the outer membrane of mitochondria and membranes of the rough endoplasmic reticulum. Recruited to the Golgi complex by the small GTPase ARF to stimulate the synthesis of phosphatidylinositol 4,5-bisphosphate (PIP2) on the Golgi complex.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp686O1820 antibody
    • FLJ30129 antibody
    • NPIK antibody
    • Phosphatidylinositol 4 kinase beta antibody
    • Phosphatidylinositol 4 kinase catalytic beta antibody
    • Phosphatidylinositol 4 kinase catalytic beta polypeptide antibody
    • Phosphatidylinositol 4 kinase wortmannin sensitive antibody
    • Phosphatidylinositol 4-kinase beta antibody
    • PI4K BETA antibody
    • PI4K-beta antibody
    • PI4K92 antibody
    • PI4KB antibody
    • PI4KB_HUMAN antibody
    • PI4Kbeta antibody
    • PI4KIIIbeta antibody
    • PIK4CB antibody
    • PtdIns 4 kinase antibody
    • PtdIns 4-kinase beta antibody
    • Type III phosphatidylinositol 4 kinase beta antibody
    see all

Images

  • All lanes : Anti-PI4 kinase beta/PI4KB antibody (ab134756) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from 293T cells at 50 µg
    Lane 4 : Whole cell lysate from Jurkat cells at 50 µg

    Developed using the ECL technique.

    Predicted band size: 91 kDa


    Exposure time: 10 seconds


    Western Blot Gel 4-8%
  • ICC/IF image of ab134756 stained Panc-1 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab134756 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Lane 1: Detection of Human PI4 kinase beta/PI4KB by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab134756 at 6 µg/mg lysate for IP.
    Lane 2: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized a downstream epitope of PI4 kinase beta/PI4KB.
    Lane 3: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.

    Subsequent Western blot detection used ab134756 at 1 µg/ml.
    Western Blot Gel 4-8%

    Developed using the ECL technique with an exposure time of 3 seconds.

References

ab134756 has not yet been referenced specifically in any publications.

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