Synthetic peptide derived from within residues 500 - 600 of Human PIAS1.
(Peptide available as ab32218.)
Our Abpromise guarantee covers the use of ab32219 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/500 - 1/2000. Detects a band of approximately 81 kDa (predicted molecular weight: 72 kDa).|
|IP||Use at an assay dependent concentration.|
|IHC-P||1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ChIP||Use at an assay dependent concentration.|
Image courtesy of Human Protein Atlas
ab32219 staining PIAS1 in human stomach. Paraffin embedded human stomach tissue was incubated with ab32219 (1/200 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab32219 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for this antibody can be found at www.proteinatlas.org
|ab32219 recognizes the full length tagged recombinant protein which has an expected molecular weight of 98 kDa.|