Product nameAnti-PIAS1 antibody [EPR2580(2)]
See all PIAS1 primary antibodies
DescriptionRabbit monoclonal [EPR2580(2)] to PIAS1
Tested applicationsSuitable for: WB, IHC-P, ICC, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human PIAS1 aa 50-150. The exact sequence is proprietary.
- Human colon tissue. HepG2, Jurkat, 293T, Daudi cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 2.10 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 72 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.|
|ICC||1/100 - 1/250.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionFunctions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling.
Tissue specificityExpressed in numerous tissues with highest level in testis.
PathwayProtein modification; protein sumoylation.
Sequence similaritiesBelongs to the PIAS family.
Contains 1 PINIT domain.
Contains 1 SAP domain.
Contains 1 SP-RING-type zinc finger.
DomainThe LXXLL motif is a transcriptional coregulator signature.
The SP-RING-type domain is required for promoting EKLF sumoylation.
Dimethylated by PRMT1 at Arg-303 in the late phase of interferon gamma (IFN-gamma) signaling, leading to preferential interaction with STAT1 and thus resulting in release of STAT1 from its target gene.
Cellular localizationNucleus speckle. Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton.
- Information by UniProt
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Lane 1 : Anti-PIAS1 antibody [EPR2580(2)] (ab109388) at 1/500 dilution
Lane 2 : Anti-GST antibody [EPR4236] (ab111947) at 1/2000 dilution
All lanes :
Recombinant Human PIAS1 protein (ab152888)
Lysates/proteins at 0.01 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted?
Lane 1: 10 seconds.
Lane 2: 1 minute.
Blocking and dilution buffer: 5% NFDM /TBST.
All lanes : Anti-PIAS1 antibody [EPR2580(2)] (ab109388) at 1/1000 dilution
Lane 1 : HepG2 cell lysates
Lane 2 : Jurkat cell lysates
Lane 3 : HEK-293 cell lysates
Lane 4 : Daudi cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 72 kDa
Immunohistochemical analysis of paraffin-embedded Human colon tissue using 1/100 ab109388.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Chanda A et al. Identification of the SUMO E3 ligase PIAS1 as a potential survival biomarker in breast cancer. PLoS One 12:e0177639 (2017). WB, IF ; Human . Read more (PubMed: 28493978) »
- Scortegagna M et al. The E3 ubiquitin ligase Siah1 regulates adrenal gland organization and aldosterone secretion. JCI Insight 2:N/A (2017). IHC . Read more (PubMed: 29212953) »