Overview

  • Product name

    Anti-PIAS1 antibody [EPR2580(2)]
    See all PIAS1 primary antibodies
  • Description

    Rabbit monoclonal [EPR2580(2)] to PIAS1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PIAS1 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Human colon tissue. HepG2, Jurkat, 293T, Daudi cell lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 72 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
ICC 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling.
    • Tissue specificity

      Expressed in numerous tissues with highest level in testis.
    • Pathway

      Protein modification; protein sumoylation.
    • Sequence similarities

      Belongs to the PIAS family.
      Contains 1 PINIT domain.
      Contains 1 SAP domain.
      Contains 1 SP-RING-type zinc finger.
    • Domain

      The LXXLL motif is a transcriptional coregulator signature.
      The SP-RING-type domain is required for promoting EKLF sumoylation.
    • Post-translational
      modifications

      Sumoylated.
      Dimethylated by PRMT1 at Arg-303 in the late phase of interferon gamma (IFN-gamma) signaling, leading to preferential interaction with STAT1 and thus resulting in release of STAT1 from its target gene.
    • Cellular localization

      Nucleus speckle. Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton.
    • Information by UniProt
    • Database links

    • Alternative names

      • AR interacting protein antibody
      • DDXBP1 antibody
      • DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1 antibody
      • DEAD/H box binding protein 1 antibody
      • DEAD/H box-binding protein 1 antibody
      • E3 SUMO-protein ligase PIAS1 antibody
      • GBP antibody
      • Gu binding protein antibody
      • Gu-binding protein antibody
      • GU/RH-II antibody
      • Pias1 antibody
      • PIAS1_HUMAN antibody
      • Protein inhibitor of activated STAT protein 1 antibody
      • Protein inhibitor of activated STAT, 1 antibody
      • RNA helicase II binding protein antibody
      • RNA helicase II-binding protein antibody
      • Zinc finger, MIZ-type containing 3 antibody
      • ZMIZ3 antibody
      see all

    Images

    • Lane 1 : Anti-PIAS1 antibody [EPR2580(2)] (ab109388) at 1/500 dilution
      Lane 2 : Anti-GST antibody [EPR4236] (ab111947) at 1/2000 dilution

      All lanes : Recombinant Human PIAS1 protein (ab152888)

      Lysates/proteins at 0.01 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 72 kDa
      Observed band size: 98 kDa
      why is the actual band size different from the predicted?



      Exposure time:

      Lane 1: 10 seconds.

      Lane 2: 1 minute.

       

      Blocking and dilution buffer: 5% NFDM /TBST.

    • All lanes : Anti-PIAS1 antibody [EPR2580(2)] (ab109388) at 1/1000 dilution

      Lane 1 : HepG2 cell lysates
      Lane 2 : Jurkat cell lysates
      Lane 3 : HEK-293 cell lysates
      Lane 4 : Daudi cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 72 kDa

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue using 1/100 ab109388.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Chanda A  et al. Identification of the SUMO E3 ligase PIAS1 as a potential survival biomarker in breast cancer. PLoS One 12:e0177639 (2017). WB, IF ; Human . Read more (PubMed: 28493978) »
    • Scortegagna M  et al. The E3 ubiquitin ligase Siah1 regulates adrenal gland organization and aldosterone secretion. JCI Insight 2:N/A (2017). IHC . Read more (PubMed: 29212953) »
    See all 8 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Western blot
    Sample
    Mouse Cell lysate - nuclear (MEF)
    Gel Running Conditions
    Reduced Denaturing (8)
    Loading amount
    20 µg
    Specification
    MEF
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Mar 07 2019

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton 5 minutes
    Specification
    HeLa
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Oct 26 2017

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Mar 31 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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