Overview

  • Product name

  • Description

    Rabbit polyclonal to PIAS2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Human PIAS2 aa 581-621.
    Sequence:

    TSVTTTSSHESSTHVSSSSSRSETGVITSSGSNIPDIISLD


    Database link: O75928

  • Positive control

    • IHC-P: Human testis tissue. ICC/IF: U-2 OS cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab244538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

IHC-P 1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'Lys-160'.
  • Tissue specificity

    Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs.
  • Pathway

    Protein modification; protein sumoylation.
  • Sequence similarities

    Belongs to the PIAS family.
    Contains 1 PINIT domain.
    Contains 1 SAP domain.
    Contains 1 SP-RING-type zinc finger.
  • Developmental stage

    Isoform 3 expression in adult testis is 14.2-fold stronger than in embryonic testis.
  • Domain

    The LXXLL motif is a transcriptional coregulator signature.
  • Post-translational
    modifications

    Sumoylated.
  • Cellular localization

    Nucleus speckle. Nucleus, PML body. Nucleus. Colocalizes at least partially with promyelocytic leukemia nuclear bodies (PML NBs) (PubMed:22406621). Colocalizes with SUMO1 in nuclear granules (By similarity).
  • Information by UniProt
  • Database links

  • Alternative names

    • Androgen receptor interacting protein 3 antibody
    • Androgen receptor-interacting protein 3 antibody
    • ARIP3 antibody
    • DAB2 interacting protein antibody
    • DAB2-interacting protein antibody
    • DIP antibody
    • E3 SUMO protein ligase PIAS2 antibody
    • E3 SUMO-protein ligase PIAS2 antibody
    • MIZ antibody
    • Miz1 antibody
    • Msx interacting zinc finger protein antibody
    • Msx-interacting zinc finger protein antibody
    • PIAS NY protein antibody
    • PIAS-NY protein antibody
    • PIAS2 antibody
    • PIAS2_HUMAN antibody
    • PIASX antibody
    • PIASX-ALPHA antibody
    • PIASX-BETA antibody
    • Protein inhibitor of activated STAT x antibody
    • Protein inhibitor of activated STAT, 2 antibody
    • Protein inhibitor of activated STAT2 antibody
    • SIZ2 antibody
    • Zinc finger, MIZ-type containing 4 antibody
    • ZMIZ4 antibody
    see all

Images

  • PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for PIAS2 (green) using ab244538 at 4 µg/ml in ICC/IF.

  • Paraffin-embedded human testis tissue stained for PIAS2 using ab244538 at 1/1000 dilution in immunohistochemical analysis.
  • Paraffin-embedded human prostate tissue stained for PIAS2 using ab244538 at 1/1000 dilution in immunohistochemical analysis.

References

ab244538 has not yet been referenced specifically in any publications.

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