Product nameAnti-PIAS3 antibody
See all PIAS3 primary antibodies
DescriptionRabbit polyclonal to PIAS3
SpecificityDetects endogenous levels of total PIAS3 protein
Tested applicationsSuitable for: WB, IHC-P, ELISA, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide derived from Internal of human PIAS3.
- Human lung carcinoma tissue 293 cells treated with UV (5 mins)
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen
Our Abpromise guarantee covers the use of ab58406 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
FunctionFunctions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678, PubMed:9388184). Sumoylates CCAR2 which promotes its interaction with SIRT1 (PubMed:25406032). Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (PubMed:24651376).
Tissue specificityWidely expressed.
PathwayProtein modification; protein sumoylation.
Sequence similaritiesBelongs to the PIAS family.
Contains 1 PINIT domain.
Contains 1 SAP domain.
Contains 1 SP-RING-type zinc finger.
DomainThe PINIT domain of PIAS3 is required for STAT3-PIAS3 interaction and for transloaction to the nucleus.
The LXXLL motif is a transcriptional coregulator signature.
Cellular localizationCytoplasm. Nucleus. Nucleus speckle. Colocalizes with MITF in the nucleus. Colocalizes with GFI1 in nuclear dots. Colocalizes with SUMO1 in nuclear granules.
- Information by UniProt
- E3 SUMO protein ligase PIAS 3 antibody
- E3 SUMO protein ligase PIAS3 antibody
- E3 SUMO-protein ligase PIAS3 antibody
Immunohistochemical analysis of paraffin embedded human lung carcinoma tissue using ab58406 at 1/50-1/100 dilution. Samples were treated -/+ peptide.
All lanes : Anti-PIAS3 antibody (ab58406) at 1/500 dilution
Lane 1 : 293 cell extract treated with UV (5 min)
Lane 2 : 293 cell extract treated with UV (5 min) with peptide
Predicted band size: 67 kDa
Observed band size: 67 kDa
This product has been referenced in:
- Iwamoto H et al. Coffee diterpenes kahweol acetate and cafestol synergistically inhibit the proliferation and migration of prostate cancer cells. Prostate N/A:N/A (2018). Read more (PubMed: 30569541) »
- Ray S et al. Suppression of STAT3 NH2 -terminal domain chemosensitizes medulloblastoma cells by activation of protein inhibitor of activated STAT3 via de-repression by microRNA-21. Mol Carcinog 57:536-548 (2018). Read more (PubMed: 29280516) »