The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
Use at 2-5 µg/mg of lysate.
Mediator of progesterone that by acting on the phospholipase A2 enzyme interferes with arachidonic acid metabolism, induces a Th2 biased immune response, and by controlling NK activity exerts an anti-abortive effect.
Expressed at highest levels in testis. Moderate expression is detected in spleen, thymus, prostate, ovary, small intestine, and colon.
Detection of PIBF by Western Blot of Immunprecipitate. ab72118 at 1 µg/ml staining PIBF in HeLa whole cell lysate immunoprecipitated using ab72118 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.