Product nameAnti-PIBF antibody
See all PIBF primary antibodies
DescriptionRabbit polyclonal to PIBF
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Dog, Rhesus monkey, Gorilla, Bat
Synthetic peptide within Human PIBF aa 735-757. The exact sequence is proprietary.
Database link: NP_006337.2
- WB: HeLa and 293T whole cell lysates. IP: HeLa whole cell lysate (ab150035).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab72118 was affinity purified using an epitope specific to PIBF immobilized on solid support.
Our Abpromise guarantee covers the use of ab72118 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionMediator of progesterone that by acting on the phospholipase A2 enzyme interferes with arachidonic acid metabolism, induces a Th2 biased immune response, and by controlling NK activity exerts an anti-abortive effect.
Tissue specificityExpressed at highest levels in testis. Moderate expression is detected in spleen, thymus, prostate, ovary, small intestine, and colon.
- Information by UniProt
- C13orf24 antibody
- KIAA1008 antibody
- PIBF 1 antibody
All lanes : Anti-PIBF antibody (ab72118) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 90 kDa
Observed band size: 90 kDa
Exposure time: 30 seconds
Detection of PIBF by Western Blot of Immunprecipitate.
ab72118 at 1 µg/ml staining PIBF in HeLa whole cell lysate immunoprecipitated using ab72118 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
ab72118 has not yet been referenced specifically in any publications.