Anti-PICALM antibody (ab106409)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-PICALM antibody
See all PICALM primary antibodies -
Description
Rabbit polyclonal to PICALM -
Host species
Rabbit -
Specificity
At least three isoforms of PICALM are known to exist; ab106409 will recognize all three. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
A 15 amino acid peptide from near the amino terminus of Human PICALM (NP_009097).
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Positive control
- EL4 cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab106409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 20 µg/ml. | |
| WB | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 71 kDa. |
Target
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Function
Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. -
Tissue specificity
Expressed in all tissues examined. -
Involvement in disease
Note=A chromosomal aberration involving PICALM is found in diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with MLLT10. -
Sequence similarities
Contains 1 ENTH (epsin N-terminal homology) domain. -
Cellular localization
Membrane > clathrin-coated pit. Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle. Colocalized with clathrin in the Golgi area. - Information by UniProt
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Database links
- Entrez Gene: 8301 Human
- Entrez Gene: 233489 Mouse
- Entrez Gene: 89816 Rat
- Omim: 603025 Human
- SwissProt: Q13492 Human
- SwissProt: Q7M6Y3 Mouse
- SwissProt: O55012 Rat
- Unigene: 163893 Human
see all -
Alternative names
- CALM antibody
- Clathrin assembly lymphoid myeloid leukemia antibody
- Clathrin assembly lymphoid myeloid leukemia protein antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-PICALM antibody (ab106409)Immunofluorescence of PICALM in Human brain tissue with ab106409 at 20 ug/mL.
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Immunocytochemistry/ Immunofluorescence - Anti-PICALM antibody (ab106409)This image is courtesy of Dr. Ruma Raha-Chowdhury, Cambridge University, United Kingdom.ICC/IF image of PICALM staining on human blood smear using ab106409 (1:200). The blood film was fixed in paraformaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The cells were then blocked using 10% donkey serum for 1 hour at 24°C. ab106409 was diluted 1200 using and incubated with the cells for 4 hours at 24°C. The secondary antibody used was donkey polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000)
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Western blot - Anti-PICALM antibody (ab106409)All lanes : Anti-PICALM antibody (ab106409) at 1 µg/ml
Lane 1 : EL4 cell lysate
Lane 2 : EL4 cell lysate with immunizing peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 71 kDa
Datasheets and documents
References
ab106409 has not yet been referenced specifically in any publications.
