- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-PICALM antibody
See all PICALM primary antibodies -
DescriptionRabbit polyclonal to PICALM
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Host speciesRabbit
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SpecificityAt least three isoforms of PICALM are known to exist; ab106409 will recognize all three.
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Tested applicationsSuitable for: ICC/IF, WBmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
A 15 amino acid peptide from near the amino terminus of Human PICALM (NP_009097).
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Positive control
- EL4 cell lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months).
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab106409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 20 µg/ml. | |
| WB | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 71 kDa. |
Target
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FunctionAssembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction.
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Tissue specificityExpressed in all tissues examined.
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Involvement in diseaseNote=A chromosomal aberration involving PICALM is found in diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with MLLT10.
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Sequence similaritiesContains 1 ENTH (epsin N-terminal homology) domain.
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Cellular localizationMembrane > clathrin-coated pit. Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle. Colocalized with clathrin in the Golgi area.
- Information by UniProt
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Database links
- Entrez Gene: 8301 Human
- Entrez Gene: 233489 Mouse
- Entrez Gene: 89816 Rat
- Omim: 603025 Human
- SwissProt: Q13492 Human
- SwissProt: Q7M6Y3 Mouse
- SwissProt: O55012 Rat
- Unigene: 163893 Human
see all -
Alternative names
- CALM antibody
- Clathrin assembly lymphoid myeloid leukemia antibody
- Clathrin assembly lymphoid myeloid leukemia protein antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-PICALM antibody (ab106409)Immunofluorescence of PICALM in Human brain tissue with ab106409 at 20 ug/mL.
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Immunocytochemistry/ Immunofluorescence - Anti-PICALM antibody (ab106409)This image is courtesy of Dr. Ruma Raha-Chowdhury, Cambridge University, United Kingdom.ICC/IF image of PICALM staining on blood smear using ab106409 (1:200). The blood film was fixed in paraformaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The cells were then blocked using 10% donkey serum for 1 hour at 24°C. ab106409 was diluted 1200 using and incubated with the cells for 4 hours at 24°C. The secondary antibody used was donkey polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000)
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Western blot - Anti-PICALM antibody (ab106409)All lanes : Anti-PICALM antibody (ab106409) at 1 µg/ml
Lane 1 : EL4 cell lysate
Lane 2 : EL4 cell lysate with immunizing peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 71 kDa
References
ab106409 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"