Anti-PICALM antibody (ab106409)
Key features and details
- Rabbit polyclonal to PICALM
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-PICALM antibody
See all PICALM primary antibodies -
Description
Rabbit polyclonal to PICALM -
Host species
Rabbit -
Specificity
At least three isoforms of PICALM are known to exist; ab106409 will recognize all three. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
A 15 amino acid peptide from near the amino terminus of Human PICALM (NP_009097).
-
Positive control
- EL4 cell lysate
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab106409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 20 µg/ml. | |
| WB | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 71 kDa. |
Target
-
Function
Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. -
Tissue specificity
Expressed in all tissues examined. -
Involvement in disease
Note=A chromosomal aberration involving PICALM is found in diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with MLLT10. -
Sequence similarities
Contains 1 ENTH (epsin N-terminal homology) domain. -
Cellular localization
Membrane > clathrin-coated pit. Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle. Colocalized with clathrin in the Golgi area. - Information by UniProt
-
Database links
- Entrez Gene: 8301 Human
- Entrez Gene: 233489 Mouse
- Entrez Gene: 89816 Rat
- Omim: 603025 Human
- SwissProt: Q13492 Human
- SwissProt: Q7M6Y3 Mouse
- SwissProt: O55012 Rat
- Unigene: 163893 Human
see all -
Alternative names
- CALM antibody
- Clathrin assembly lymphoid myeloid leukemia antibody
- Clathrin assembly lymphoid myeloid leukemia protein antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-PICALM antibody (ab106409)
Immunofluorescence of PICALM in Human brain tissue with ab106409 at 20 ug/mL.
-
Immunocytochemistry/ Immunofluorescence - Anti-PICALM antibody (ab106409)This image is courtesy of Dr. Ruma Raha-Chowdhury, Cambridge University, United Kingdom.ICC/IF image of PICALM staining on human blood smear using ab106409 (1:200). The blood film was fixed in paraformaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The cells were then blocked using 10% donkey serum for 1 hour at 24°C. ab106409 was diluted 1200 using and incubated with the cells for 4 hours at 24°C. The secondary antibody used was donkey polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000)
-
Western blot - Anti-PICALM antibody (ab106409)All lanes : Anti-PICALM antibody (ab106409) at 1 µg/ml
Lane 1 : EL4 cell lysate
Lane 2 : EL4 cell lysate with immunizing peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 71 kDa
Protocols
Datasheets and documents
References (0)
ab106409 has not yet been referenced specifically in any publications.
