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Anti-PICALM antibody (ab106409)

  • Datasheet
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Immunocytochemistry/ Immunofluorescence - Anti-PICALM antibody (ab106409)
  • Immunocytochemistry/ Immunofluorescence - Anti-PICALM antibody (ab106409)
  • Western blot - Anti-PICALM antibody (ab106409)

Key features and details

  • Rabbit polyclonal to PICALM
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-PICALM antibody
    See all PICALM primary antibodies
  • Description

    Rabbit polyclonal to PICALM
  • Host species

    Rabbit
  • Specificity

    At least three isoforms of PICALM are known to exist; ab106409 will recognize all three.
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 15 amino acid peptide from near the amino terminus of Human PICALM (NP_009097).

  • Positive control

    • EL4 cell lysate
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at 4°C (stable for up to 12 months).
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • EL4 whole cell lysate (ab7183)

Applications

Our Abpromise guarantee covers the use of ab106409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 71 kDa.

Target

  • Function

    Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction.
  • Tissue specificity

    Expressed in all tissues examined.
  • Involvement in disease

    Note=A chromosomal aberration involving PICALM is found in diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with MLLT10.
  • Sequence similarities

    Contains 1 ENTH (epsin N-terminal homology) domain.
  • Cellular localization

    Membrane > clathrin-coated pit. Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle. Colocalized with clathrin in the Golgi area.
  • Target information above from: UniProt accession Q13492 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8301 Human
    • Entrez Gene: 233489 Mouse
    • Entrez Gene: 89816 Rat
    • Omim: 603025 Human
    • SwissProt: Q13492 Human
    • SwissProt: Q7M6Y3 Mouse
    • SwissProt: O55012 Rat
    • Unigene: 163893 Human
    • Unigene: 235175 Mouse
    • Unigene: 393563 Mouse
    • Unigene: 24871 Rat
    see all
  • Alternative names

    • CALM antibody
    • Clathrin assembly lymphoid myeloid leukemia antibody
    • Clathrin assembly lymphoid myeloid leukemia protein antibody
    • CLTH antibody
    • LAP antibody
    • Phosphatidylinositol-binding clathrin assembly protein antibody
    • PICAL_HUMAN antibody
    • PICALM antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PICALM antibody (ab106409)
    Immunocytochemistry/ Immunofluorescence - Anti-PICALM antibody (ab106409)

    Immunofluorescence of PICALM in Human brain tissue with ab106409 at 20 ug/mL.

  • Immunocytochemistry/ Immunofluorescence - Anti-PICALM antibody (ab106409)
    Immunocytochemistry/ Immunofluorescence - Anti-PICALM antibody (ab106409)This image is courtesy of Dr. Ruma Raha-Chowdhury, Cambridge University, United Kingdom.

    ICC/IF image of PICALM staining on human blood smear using ab106409 (1:200). The blood film was fixed in paraformaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The cells were then blocked using 10% donkey serum for 1 hour at 24°C. ab106409 was diluted 1200 using and incubated with the cells for 4 hours at 24°C. The secondary antibody used was donkey polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000)

  • Western blot - Anti-PICALM antibody (ab106409)
    Western blot - Anti-PICALM antibody (ab106409)
    All lanes : Anti-PICALM antibody (ab106409) at 1 µg/ml

    Lane 1 : EL4 cell lysate
    Lane 2 : EL4 cell lysate with immunizing peptide

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 71 kDa

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab106409? Please let us know so that we can cite the reference in this datasheet.

    ab106409 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-PICALM antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (10)
    Sample
    Human Serum (Serum)
    Specification
    Serum
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Aug 14 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-PICALM antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Human Cell (Blood smear)
    Specification
    Blood smear
    Permeabilization
    Yes - 0.1% TritonX in 0.1% PBS
    Fixative
    Paraformaldehyde
    Read More

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Sep 27 2013

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