Overview

  • Product name

  • Description

    Rabbit polyclonal to PICALM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human PICALM aa 602-652. The exact sequence is proprietary.
    Database link: Q13492

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab226411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 71 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction.
    • Tissue specificity

      Expressed in all tissues examined.
    • Involvement in disease

      Note=A chromosomal aberration involving PICALM is found in diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with MLLT10.
    • Sequence similarities

      Contains 1 ENTH (epsin N-terminal homology) domain.
    • Cellular localization

      Membrane > clathrin-coated pit. Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle. Colocalized with clathrin in the Golgi area.
    • Information by UniProt
    • Database links

    • Alternative names

      • CALM antibody
      • Clathrin assembly lymphoid myeloid leukemia antibody
      • Clathrin assembly lymphoid myeloid leukemia protein antibody
      • CLTH antibody
      • LAP antibody
      • Phosphatidylinositol-binding clathrin assembly protein antibody
      • PICAL_HUMAN antibody
      • PICALM antibody
      see all

    Images

    • All lanes : Anti-PICALM antibody (ab226411) at 0.1 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
      Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
      Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

      Lysates/proteins at 15 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 71 kDa


      Exposure time: 30 seconds


      Cell lysates were prepared using NETN lysis buffer.

    References

    ab226411 has not yet been referenced specifically in any publications.

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