Key features and details
- Rabbit polyclonal to PICALM
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PICALM antibody
See all PICALM primary antibodies
DescriptionRabbit polyclonal to PICALM
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human PICALM aa 602-652. The exact sequence is proprietary.
Database link: Q13492
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226411 was affinity purified using an epitope specific to PICALM immobilized on solid support.
Our Abpromise guarantee covers the use of ab226411 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 71 kDa.|
FunctionAssembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction.
Tissue specificityExpressed in all tissues examined.
Involvement in diseaseNote=A chromosomal aberration involving PICALM is found in diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with MLLT10.
Sequence similaritiesContains 1 ENTH (epsin N-terminal homology) domain.
Cellular localizationMembrane > clathrin-coated pit. Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle. Colocalized with clathrin in the Golgi area.
- Information by UniProt
- CALM antibody
- Clathrin assembly lymphoid myeloid leukemia antibody
- Clathrin assembly lymphoid myeloid leukemia protein antibody
All lanes : Anti-PICALM antibody (ab226411) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 71 kDa
Exposure time: 30 seconds
Cell lysates were prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226411 has not yet been referenced specifically in any publications.