Overview

  • Product name
    Anti-PICALM antibody [EPR12177]
    See all PICALM primary antibodies
  • Description
    Rabbit monoclonal [EPR12177] to PICALM
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human PICALM aa 550-650 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q13492

  • Positive control
    • HepG2, HeLa, A431 and Human fetal brain lysates; Human placenta and breast tissues; HeLa cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab172962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 66-71 kDa (predicted molecular weight: 71 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction.
    • Tissue specificity
      Expressed in all tissues examined.
    • Involvement in disease
      Note=A chromosomal aberration involving PICALM is found in diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with MLLT10.
    • Sequence similarities
      Contains 1 ENTH (epsin N-terminal homology) domain.
    • Cellular localization
      Membrane > clathrin-coated pit. Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle. Colocalized with clathrin in the Golgi area.
    • Information by UniProt
    • Database links
    • Alternative names
      • CALM antibody
      • Clathrin assembly lymphoid myeloid leukemia antibody
      • Clathrin assembly lymphoid myeloid leukemia protein antibody
      • CLTH antibody
      • LAP antibody
      • Phosphatidylinositol-binding clathrin assembly protein antibody
      • PICAL_HUMAN antibody
      • PICALM antibody
      see all

    Images

    • All lanes : Anti-PICALM antibody [EPR12177] (ab172962) at 1/1000 dilution

      Lane 1 : HepG2 lysate
      Lane 2 : HeLa lysate
      Lane 3 : A431 lysate
      Lane 4 : Human fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 71 kDa

    • Immunohistochemical analysis of paraffin-embedded Human Placenta tissue labeling PICALM with ab172962 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human Breast tissue labeling PICALM with ab172962 at 1/50 dilution.

    • Immunofluorescent analysis of HeLa cells labeling PICALM with ab172962 at 1/100 dilution.

    References

    This product has been referenced in:
    • Ramachandran S  et al. Cis-acting single nucleotide polymorphisms alter MicroRNA-mediated regulation of human brain-expressed transcripts. Hum Mol Genet 25:4939-4950 (2016). Read more (PubMed: 28171541) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry
    Sample
    African green monkey Cell (COS7 cells (Kidney))
    Permeabilization
    Yes - 0.2% Triton X100
    Specification
    COS7 cells (Kidney)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Methanol

    Abcam user community

    Verified customer

    Submitted Aug 14 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry
    Sample
    Human Cell (Hela cells (Epithelial))
    Permeabilization
    Yes - 0.2% Triton X-100
    Specification
    Hela cells (Epithelial)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Methanol

    Abcam user community

    Verified customer

    Submitted Aug 14 2017

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Brain)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jul 27 2017

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Cow Cell lysate - whole cell (Lung)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Lung
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jul 20 2017

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    African green monkey Cell lysate - whole cell (Kidney)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jul 20 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Kidney)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jul 20 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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