Overview

  • Product name

    Anti-Piccolo antibody [6H9-B6]
    See all Piccolo primary antibodies
  • Description

    Mouse monoclonal [6H9-B6] to Piccolo
  • Host species

    Mouse
  • Specificity

    No cross reactivity to Bassoon.
  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Full length protein corresponding to Mouse Piccolo.
    Database link: Q9QYX7

  • Positive control

    • WB: Rat and mouse brain tissue lysate. ICC/IF: SK-N-BE cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    6H9-B6
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 567 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF 1/100.

Target

  • Function

    May act as a scaffolding protein involved in the organization of synaptic active zones and in synaptic vesicle trafficking.
  • Sequence similarities

    Contains 2 C2 domains.
    Contains 1 PDZ (DHR) domain.
  • Domain

    C2 domain 1 is involved in binding calcium and phospholipids. Calcium binds with low affinity but with high specificity and induces a large conformational change.
  • Cellular localization

    Cell junction > synapse. Concentrated at the presynaptic side of synaptic junctions.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACZ antibody
    • Aczonin antibody
    • Brain-derived HLMN protein antibody
    • DKFZp779G1236 antibody
    • KIAA0559 antibody
    • Multidomain presynaptic cytomatrix protein antibody
    • PCLO antibody
    • PCLO_HUMAN antibody
    • Piccolo (presynaptic cytomatrix protein) antibody
    • Piccolo, mouse, homolog of antibody
    • Pico antibody
    • Presynaptic cytomatrix protein antibody
    • Protein piccolo antibody
    see all

Images

  • SK-N-BE cells labeling Piccolo using ab101654 (green) at 1/100 dilution in ICC/IF. Cells were fixed using 4% formaldehyde for 15 minutes at room temperature. Primary antibody was incubated for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 at 1/100 dilution for 1 hour at room temperature. Counterstained with Phalloidin Texas Red F-actin stain. Nuclei were stained with DAPI (blue).

  • All lanes : Anti-Piccolo antibody [6H9-B6] (ab101654) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Predicted band size: 567 kDa

References

This product has been referenced in:

  • Mateos-Hernández L  et al. Quantitative proteomics reveals Piccolo as a candidate serological correlate of recovery from Guillain-Barré syndrome. Oncotarget 7:74582-74591 (2016). ELISA ; Human . Read more (PubMed: 27776345) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your reply.

Yes, ab101654 is the only mous monoclonal antibody we have for Piccolo. It has not yet been tested with mouse samples and in IHC-P (paraffin-embedded PFA-fixed tissue sections). We cannot guarantee if and how well the antibody will work, but you can use our testing discount program to try the antibody.

For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the antibody/protein/kit at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody/protein at the full price (100%) of the antibody/protein you have tested, or a full price discount for the amount paid for the kit. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
This programapplies to ELISA kits and other kits we offer.

Please let me know if I shall send you a discount code.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Vielen Dank für Ihren Anruf. Wie versprochen hatte ich für den anti-Piccolo- Antikörper ab101654 nachgefragt, ob das Epitop kartiert worden ist. Daraufhin hat man mir die Immunogensequenz geschickt, die zur Generierung dieses Antikörpers verwendet wurde (siehe Anhang). Es handelt sich dabei um die 356 Aminosäuren lange Isoform 3 des humanen Piccoloproteins (Genbank Accession number BC001304.1, SwissProtID Q9Y6V0, http://www.uniprot.org/uniprot/Q9Y6V0#Q9Y6V0-3). Ein Alignment der verschiedenen Isoformen und Sequenzen zeigt, dass Isoform 3 den C-terminalen Aminosäuren (ab ca Position 4500) der Isoformen 2, 5 und 6 entspricht. Schließt dies Ihre geplante Mutation ein und würde sich der Antikörper damit für Ihre Vorhaben eignen? Sie erwähnten auch, dass Sie eventuell IP-Experimente durchführen möchten. Unseres Wissens nach wurde der Antikörper ab101654 bisher noch nicht in IP getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen daher zur Zeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen kostenlosen primären Antikörper Ihrer Wahl, wenn Sie uns ein Abreview mit dem Testresultat zusenden (https://www.abcam.com/collaborationdiscount). Bei Interesse kann ich Ihnen gerne nähere Informationen zukommen lassen. Wegen der Immunogensequenzen der anderen beiden Piccolo- Antikörper ab76186 und ab77496 habe mich mit dem Labor, welches diese Antikörper herstellt, in Verbindung gesetzt, warte aber immer noch auf eine Antwort. Ich werde mich wieder bei Ihnen melden, sobald ich diese bekommen habe. Bitte entschuldigen Sie die Verzögerung und vielen Dank für Ihre Geduld.

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