Key features and details
- Rabbit polyclonal to Piccolo
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Piccolo antibody
See all Piccolo primary antibodies
DescriptionRabbit polyclonal to Piccolo
SpecificityNo cross-reactivity to Bassoon. Multiple isoforms can be detected.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Full length Human Piccolo protein (BC001304.1).
- Mouse and Rat brain lysate; Nuromuscular junction muscle samples from adult mouse.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferConstituent: PBS
0.09% Sodium Azide
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab110427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 567 kDa. For large isoforms reduce dilution.|
FunctionMay act as a scaffolding protein involved in the organization of synaptic active zones and in synaptic vesicle trafficking.
Sequence similaritiesContains 2 C2 domains.
Contains 1 PDZ (DHR) domain.
DomainC2 domain 1 is involved in binding calcium and phospholipids. Calcium binds with low affinity but with high specificity and induces a large conformational change.
Cellular localizationCell junction > synapse. Concentrated at the presynaptic side of synaptic junctions.
- Information by UniProt
- ACZ antibody
- Aczonin antibody
- Brain-derived HLMN protein antibody
All lanes : Anti-Piccolo antibody (ab110427) at 1 µg/ml
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 567 kDa
ab110427 at 1/400 staining Piccolo in neuromuscular junction of whole muscle sample from adult mouse by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Secondary used was Alexa Fluor® 488 goat anti-mouse IgG, IgM (H+L). Red staining was an antibody directed against BTX.
ab110427 has been referenced in 1 publication.
- Song SY et al. Environmental Enrichment Upregulates Striatal Synaptic Vesicle-Associated Proteins and Improves Motor Function. Front Neurol 9:465 (2018). PubMed: 30061854