Product namePiceatannol, Syk tyrosine kinase inhibitor
DescriptionSyk tyrosine kinase inhibitor
Biological descriptionSyk tyrosine kinase inhibitor (Ki = 15 μM at Syk). Shows multiple biological activities such as anti-inflammatory, antiproliferative and immunomodulatory effects.
Storage instructionsStore at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in DMSO to 100 mM and in ethanol to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Our Abpromise guarantee covers the use of ab120722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab119954 staining CBL in A431 cells treated with piceatannol (ab120722), by ICC/IF. Decrease in CBL expression correlates with increased concentration of piceatannol, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120722 (piceatannol) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab119954 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab120722 has been referenced in 3 publications.
- Lu YQ et al. NLRP3 inflammasome activation results in liver inflammation and fibrosis in mice infected with Schistosoma japonicum in a Syk-dependent manner. Sci Rep 7:8120 (2017). PubMed: 28808303
- Muramatsu D et al. Aureobasidium pullulans produced ß-glucan is effective to enhance Kurosengoku soybean extract induced Thrombospondin-1 expression. Sci Rep 7:2831 (2017). PubMed: 28588201
- Chan CN et al. Potent Inhibition of HIV-1 Replication in Resting CD4 T Cells by Resveratrol and Pterostilbene. Antimicrob Agents Chemother 61:N/A (2017). PubMed: 28652233