Overview

  • Product name

  • Description

    Rabbit polyclonal to PICH
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Fusion protein corresponding to Human PICH (C terminal). C-terminal 250 amino acids. The identity of the protein fusion partner is GST.
    Database link: BC008808

  • Positive control

    • Human cervical cancer and colon cancer tissue
  • General notes

     This product was previously labelled as ERCC6L

     

Properties

Applications

Our Abpromise guarantee covers the use of ab197925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.

Target

  • Function

    DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase.
  • Sequence similarities

    Belongs to the SNF2/RAD54 helicase family.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 2 TPR repeats.
  • Post-translational
    modifications

    Phosphorylation by PLK1 prevents the association with chromosome arms and restricts its localization to the kinetochore-centromere region.
  • Cellular localization

    Chromosome > centromere. Chromosome > centromere > kinetochore. Localizes to kinetochores, inner centromeres and thin threads connecting separating chromosomes even during anaphase. In prometaphase cells, it mostly concentrates in between kinetochores. In metaphase, it localizes to numerous thin threads that stretch between sister kinetochores of the aligned chromosomes and are composed of catenated centromeric DNA. Evolution from inner centromeres to thin threads takes place in response to tension. Resolution of thin threads requires topoisomerase 2-alpha (TOP2A) after anaphase onset.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATP dependent helicase ERCC6 like antibody
    • ATP-dependent helicase ERCC6-like antibody
    • DNA excision repair protein ERCC 6 like antibody
    • DNA excision repair protein ERCC-6-like antibody
    • ERC6L_HUMAN antibody
    • ERCC 6L antibody
    • Ercc6l antibody
    • Excision repair cross complementing rodent repair deficiency complementation group 6 like antibody
    • Excision repair protein ERCC6 like antibody
    • FLJ20105 antibody
    • MGC131695 antibody
    • PICH antibody
    • Plk1 interacting checkpoint helicase antibody
    • PLK1-interacting checkpoint helicase antibody
    • SNF2/RAD54 family protein antibody
    • Tumor antigen BJ HCC 15 antibody
    • Tumor antigen BJ-HCC-15 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling PICH using ab197925 at 1/30 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling PICH using ab197925 at 1/30 dilution.

References

ab197925 has not yet been referenced specifically in any publications.

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