Key features and details
- Rabbit polyclonal to PICH
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PICH antibody
See all PICH primary antibodies
DescriptionRabbit polyclonal to PICH
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Fusion protein corresponding to Human PICH (C terminal). C-terminal 250 amino acids. The identity of the protein fusion partner is GST.
Database link: BC008808
- Human cervical cancer and colon cancer tissue
This product was previously labelled as ERCC6L
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab197925 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200.|
FunctionDNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase.
Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 2 TPR repeats.
modificationsPhosphorylation by PLK1 prevents the association with chromosome arms and restricts its localization to the kinetochore-centromere region.
Cellular localizationChromosome > centromere. Chromosome > centromere > kinetochore. Localizes to kinetochores, inner centromeres and thin threads connecting separating chromosomes even during anaphase. In prometaphase cells, it mostly concentrates in between kinetochores. In metaphase, it localizes to numerous thin threads that stretch between sister kinetochores of the aligned chromosomes and are composed of catenated centromeric DNA. Evolution from inner centromeres to thin threads takes place in response to tension. Resolution of thin threads requires topoisomerase 2-alpha (TOP2A) after anaphase onset.
- Information by UniProt
- ATP dependent helicase ERCC6 like antibody
- ATP-dependent helicase ERCC6-like antibody
- DNA excision repair protein ERCC 6 like antibody
ab197925 has not yet been referenced specifically in any publications.