Key features and details
- Mouse polyclonal to PICH
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PICH antibody
See all PICH primary antibodies
DescriptionMouse polyclonal to PICH
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full length protein corresponding to Human PICH. (AAH08808.1)
- Hela cell lysate, PICH transfected 293T cell lysate.
This product was previously labelled as ERCC6L
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab88560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 141 kDa.|
FunctionDNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase.
Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 2 TPR repeats.
modificationsPhosphorylation by PLK1 prevents the association with chromosome arms and restricts its localization to the kinetochore-centromere region.
Cellular localizationChromosome > centromere. Chromosome > centromere > kinetochore. Localizes to kinetochores, inner centromeres and thin threads connecting separating chromosomes even during anaphase. In prometaphase cells, it mostly concentrates in between kinetochores. In metaphase, it localizes to numerous thin threads that stretch between sister kinetochores of the aligned chromosomes and are composed of catenated centromeric DNA. Evolution from inner centromeres to thin threads takes place in response to tension. Resolution of thin threads requires topoisomerase 2-alpha (TOP2A) after anaphase onset.
- Information by UniProt
- ATP dependent helicase ERCC6 like antibody
- ATP-dependent helicase ERCC6-like antibody
- DNA excision repair protein ERCC 6 like antibody
All lanes : Anti-PICH antibody (ab88560) at 1 µg/ml
Lane 1 : PICH transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 141 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Anti-PICH antibody (ab88560) at 1 µg/ml + Hela cell lysate at 50 µg
Predicted band size: 141 kDa
Observed band size: 141 kDa
Additional bands at: 57 kDa. We are unsure as to the identity of these extra bands.
ab88560 has been referenced in 1 publication.
- Panier S et al. SLX4IP Antagonizes Promiscuous BLM Activity during ALT Maintenance. Mol Cell 76:27-43.e11 (2019). PubMed: 31447390