Product nameAnti-PICK1 antibody [EPR4129(2)]
See all PICK1 primary antibodies
DescriptionRabbit monoclonal [EPR4129(2)] to PICK1
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human PICK1 (N terminal). The exact sequence is proprietary.
- WB: Fetal brain, HeLa, LnCaP and SH-SY5Y cell lysates IP: HeLa whole cell lysate
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109249 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionProbable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate heteromeric ACCN3/ACCN2 channel.
Sequence similaritiesContains 1 AH domain.
Contains 1 PDZ (DHR) domain.
modificationsPhosphorylation at Thr-82 appears to inhibit the interaction with AMPA receptors.
Cellular localizationCytoplasm > perinuclear region. Cell junction > synapse. Also present at excitatory synapses.
- Information by UniProt
- dJ1039K5 antibody
- MGC15204 antibody
- OTTHUMP00000028509 antibody
All lanes : Anti-PICK1 antibody [EPR4129(2)] (ab109249) at 1/1000 dilution
Lane 1 : Fetal brain tissue lysate
Lane 2 : HeLa cell lysate
Lane 3 : LnCaP cell lysate
Lane 4 : SH SY5Y cell lysate
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
ab109249 (purified) at 1/30 dilution (20 µg/ml) immunoprecipitating PICK1 in HeLa whole cell lysate.
Lane 1 (input): HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab109249 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109249 in HeLa whole cell lysate
For western blotting, ab227648 (purified at 1/500 dilution (0.012 µg/ml) and veriBlot for IP secondary antibody (HRP) (ab131366) at 1/1000 dilution were used.
Blocking and diluting buffer: 5% NFDM /TBST .
ab109249 has not yet been referenced specifically in any publications.