Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4129(2)] to PICK1
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Product nameAnti-PICK1 antibody [EPR4129(2)]
See all PICK1 primary antibodies
DescriptionRabbit monoclonal [EPR4129(2)] to PICK1
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human PICK1 (N terminal). The exact sequence is proprietary.
- WB: Fetal brain, HeLa, LnCaP and SH-SY5Y cell lysates IP: HeLa whole cell lysate
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109249 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).|
FunctionProbable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate heteromeric ACCN3/ACCN2 channel.
Sequence similaritiesContains 1 AH domain.
Contains 1 PDZ (DHR) domain.
modificationsPhosphorylation at Thr-82 appears to inhibit the interaction with AMPA receptors.
Cellular localizationCytoplasm > perinuclear region. Cell junction > synapse. Also present at excitatory synapses.
- Information by UniProt
- dJ1039K5 antibody
- MGC15204 antibody
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All lanes : Anti-PICK1 antibody [EPR4129(2)] (ab109249) at 1/1000 dilution
Lane 1 : Fetal brain tissue lysate
Lane 2 : HeLa cell lysate
Lane 3 : LnCaP cell lysate
Lane 4 : SH SY5Y cell lysate
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
ab109249 (purified) at 1/30 dilution (20 µg/ml) immunoprecipitating PICK1 in HeLa whole cell lysate.
Lane 1 (input): HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab109249 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109249 in HeLa whole cell lysate
For western blotting, ab227648 (purified at 1/500 dilution (0.012 µg/ml) and veriBlot for IP secondary antibody (HRP) (ab131366) at 1/1000 dilution were used.
Blocking and diluting buffer: 5% NFDM /TBST .
ab109249 has not yet been referenced specifically in any publications.