PicoProbe D-Lactate Assay Kit (Fluorometric) (ab174096)

Overview

  • Product name
    PicoProbe D-Lactate Assay Kit (Fluorometric)
    See all D-Lactate kits
  • Detection method
    Fluorescent
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue, Adherent cells, Suspension cells
  • Assay type
    Quantitative
  • Sensitivity
    >= 0.1 µM
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mammals
  • Product overview

    Abcam's PicoProbe D-Lactate Assay Kit (Fluorometric) (ab174096) offers simplicity, sensitivity, and can be adapted to high-throughput research. The assay enzymatically oxidizes D-Lactate generating a fluorescent signal (Ex/Em = 535/587 nm). The signal is directly proportional to the amount of D-Lactate. The assay kit can detect D-Lactate as low as 0.1 µM in a variety of samples.

     

    Visit our FAQs page for tips and troubleshooting.

  • Notes

    D-Lactate is the result of anaerobic glycolysis by microorganisms in the gastrointestinal system, and the product of detoxification of methylglyoxal by the glyoxalase system. The presence of abnormal levels of D-Lactate has been linked to a series of pathological conditions, such as diabetes, and appendicitis.

  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    D-Lactate Assay Buffer WM 1 x 25ml
    D-Lactate Enzyme Mix (Lyophilised) Green 1 vial
    D-Lactate Standard (100 mM) Yellow 1 x 0.1ml
    D-Lactate Substrate Mix (Lyophilised) Red 1 vial
    PicoProbe Blue 1 x 0.4ml
  • Research areas
  • Relevance
    D-Lactate production in mammals, mainly due to the glyoxalase pathway, is extremely low, with normal serum concentrations in the nano to micromolar range. Typically, elevated D-lactate levels which can rise to millimolar levels, are due to bacterial infection or short bowel syndrome in humans. Abnormally high concentrations of D-lactate are considered indicative of sepsis, ischemia or trauma. Due to slow metabolism and excretion, high D-lactate can cause acidosis and encephalopathy.
  • Alternative names
    • D-Lactic acid

Images

  • D-Lactate Standard curve. This assay specifically detected D-Lactate (blue diamonds) but not L-Lactate (red squares). This is example data only.

  • Diluted samples (1/10, non-diabetic; 1/100 diabetic) were spiked with known amounts of D-Lactate (0-200 pmol) and assayed following kit's protocol. This is example data only.

Protocols

References

ab174096 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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