Overview

  • Product name

  • Description

    Rabbit polyclonal to PICOT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human PICOT aa 83-335.

  • Positive control

    • Raji and mouse brain whole cell lysates; A431 cells; 293T and Jurkat cell lines
  • General notes

    Previously labelled as TXNL2. 

Properties

Applications

Our Abpromise guarantee covers the use of ab151430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Critical negative regulator of cardiac hypertrophy and a positive inotropic regulator (By similarity). May play a role in regulating the function of the thioredoxin system. Does not posses any thyoredoxin activity since it lacks the conserved motif that is essential for catalytic activity.
  • Tissue specificity

    Expressed in heart, spleen, testis and, to a lower extent, in thymus and peripheral blood leukocytes. Weakly expressed in lung, placenta, colon and small intestine.
  • Sequence similarities

    Contains 2 glutaredoxin domains.
    Contains 1 thioredoxin domain.
  • Domain

    The thioredoxin domain lacks the two redox-active cysteines. This strongly suggests that it lacks thioredoxin activity.
  • Cellular localization

    Cytoplasm > cell cortex. Cytoplasm > myofibril > sarcomere > Z line. Under the plasma membrane. After PMA stimulation, GLRX3 and PRKCQ/PKC-theta translocate to a more extended submembrane area. In the Z line, found associated with CSRP3.
  • Information by UniProt
  • Database links

  • Alternative names

    • glrx3 antibody
    • GLRX3_HUMAN antibody
    • GLRX4 antibody
    • Glutaredoxin 3 antibody
    • Glutaredoxin 4 antibody
    • Glutaredoxin-3 antibody
    • GRX3 antibody
    • GRX4 antibody
    • PICOT antibody
    • PKC interacting cousin of thioredoxin antibody
    • PKC theta interacting protein antibody
    • PKC-interacting cousin of thioredoxin antibody
    • PKC-theta-interacting protein antibody
    • PKCq interacting protein antibody
    • PKCq-interacting protein antibody
    • Thioredoxin like protein 2 antibody
    • Thioredoxin-like protein 2 antibody
    • TXNL2 antibody
    • TXNL3 antibody
    see all

Images

  • Anti-PICOT antibody (ab151430) at 1/10000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 37 kDa



    10% SDS PAGE
  • Anti-PICOT antibody (ab151430) at 1/1000 dilution + Mouse brain whole cell lyate at 50 µg

    Predicted band size: 37 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed A431 cells labeling PICOT with ab151430 at 1/200 dilution. Lower image shows cells co-stained with Hosechst 33342.

References

ab151430 has not yet been referenced specifically in any publications.

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