Overview

  • Product name

  • Description

    Rabbit polyclonal to PICOT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide within Human PICOT aa 285-335. The exact sequence is proprietary.
    Database link: O76003

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH-3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
  • General notes

    Previously labelled as TXNL2. 

Properties

Applications

Our Abpromise guarantee covers the use of ab226396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 37 kDa.

Target

  • Function

    Critical negative regulator of cardiac hypertrophy and a positive inotropic regulator (By similarity). May play a role in regulating the function of the thioredoxin system. Does not posses any thyoredoxin activity since it lacks the conserved motif that is essential for catalytic activity.
  • Tissue specificity

    Expressed in heart, spleen, testis and, to a lower extent, in thymus and peripheral blood leukocytes. Weakly expressed in lung, placenta, colon and small intestine.
  • Sequence similarities

    Contains 2 glutaredoxin domains.
    Contains 1 thioredoxin domain.
  • Domain

    The thioredoxin domain lacks the two redox-active cysteines. This strongly suggests that it lacks thioredoxin activity.
  • Cellular localization

    Cytoplasm > cell cortex. Cytoplasm > myofibril > sarcomere > Z line. Under the plasma membrane. After PMA stimulation, GLRX3 and PRKCQ/PKC-theta translocate to a more extended submembrane area. In the Z line, found associated with CSRP3.
  • Information by UniProt
  • Database links

  • Alternative names

    • glrx3 antibody
    • GLRX3_HUMAN antibody
    • GLRX4 antibody
    • Glutaredoxin 3 antibody
    • Glutaredoxin 4 antibody
    • Glutaredoxin-3 antibody
    • GRX3 antibody
    • GRX4 antibody
    • PICOT antibody
    • PKC interacting cousin of thioredoxin antibody
    • PKC theta interacting protein antibody
    • PKC-interacting cousin of thioredoxin antibody
    • PKC-theta-interacting protein antibody
    • PKCq interacting protein antibody
    • PKCq-interacting protein antibody
    • Thioredoxin like protein 2 antibody
    • Thioredoxin-like protein 2 antibody
    • TXNL2 antibody
    • TXNL3 antibody
    see all

Images

  • All lanes : Anti-PICOT antibody (ab226396) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 37 kDa


    Exposure time: 10 seconds


    Lysates prepared using NETN lysis buffer.

  • PICOT was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226396 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226396 at 0.4 µg/ml.

    Lane 1: ab226396 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab226396 has not yet been referenced specifically in any publications.

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