Key features and details
- Rabbit polyclonal to PICOT
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PICOT antibody
See all PICOT primary antibodies
DescriptionRabbit polyclonal to PICOT
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan
Synthetic peptide within Human PICOT aa 285-335. The exact sequence is proprietary.
Database link: O76003
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH-3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Previously labelled as TXNL2.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226396 was affinity purified using an epitope specific to PICOT immobilized on solid support.
Our Abpromise guarantee covers the use of ab226396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 37 kDa.|
FunctionCritical negative regulator of cardiac hypertrophy and a positive inotropic regulator (By similarity). May play a role in regulating the function of the thioredoxin system. Does not posses any thyoredoxin activity since it lacks the conserved motif that is essential for catalytic activity.
Tissue specificityExpressed in heart, spleen, testis and, to a lower extent, in thymus and peripheral blood leukocytes. Weakly expressed in lung, placenta, colon and small intestine.
Sequence similaritiesContains 2 glutaredoxin domains.
Contains 1 thioredoxin domain.
DomainThe thioredoxin domain lacks the two redox-active cysteines. This strongly suggests that it lacks thioredoxin activity.
Cellular localizationCytoplasm > cell cortex. Cytoplasm > myofibril > sarcomere > Z line. Under the plasma membrane. After PMA stimulation, GLRX3 and PRKCQ/PKC-theta translocate to a more extended submembrane area. In the Z line, found associated with CSRP3.
- Information by UniProt
- glrx3 antibody
- GLRX3_HUMAN antibody
- GLRX4 antibody
All lanes : Anti-PICOT antibody (ab226396) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 10 seconds
Lysates prepared using NETN lysis buffer.
PICOT was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226396 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226396 at 0.4 µg/ml.
Lane 1: ab226396 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226396 has not yet been referenced specifically in any publications.