Anti-PICOT antibody (ab226396)
Key features and details
- Rabbit polyclonal to PICOT
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PICOT antibody -
Description
Rabbit polyclonal to PICOT -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan -
Immunogen
Synthetic peptide within Human PICOT aa 285-335. The exact sequence is proprietary.
Database link: O76003 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH-3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab226396 was affinity purified using an epitope specific to PICOT immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-10 µg/mg of lysate.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 37 kDa.
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Notes |
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IP
Use at 2-10 µg/mg of lysate. |
WB
1/2000 - 1/10000. Predicted molecular weight: 37 kDa. |
Target
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Function
Critical negative regulator of cardiac hypertrophy and a positive inotropic regulator (By similarity). May play a role in regulating the function of the thioredoxin system. Does not posses any thyoredoxin activity since it lacks the conserved motif that is essential for catalytic activity. -
Tissue specificity
Expressed in heart, spleen, testis and, to a lower extent, in thymus and peripheral blood leukocytes. Weakly expressed in lung, placenta, colon and small intestine. -
Sequence similarities
Contains 2 glutaredoxin domains.
Contains 1 thioredoxin domain. -
Domain
The thioredoxin domain lacks the two redox-active cysteines. This strongly suggests that it lacks thioredoxin activity. -
Cellular localization
Cytoplasm > cell cortex. Cytoplasm > myofibril > sarcomere > Z line. Under the plasma membrane. After PMA stimulation, GLRX3 and PRKCQ/PKC-theta translocate to a more extended submembrane area. In the Z line, found associated with CSRP3. - Information by UniProt
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Database links
- Entrez Gene: 450828 Chimpanzee
- Entrez Gene: 511528 Cow
- Entrez Gene: 477869 Dog
- Entrez Gene: 10539 Human
- Entrez Gene: 30926 Mouse
- Entrez Gene: 100436050 Orangutan
- Entrez Gene: 100511783 Pig
- Entrez Gene: 100344020 Rabbit
see all -
Alternative names
- glrx3 antibody
- GLRX3_HUMAN antibody
- GLRX4 antibody
see all
Images
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All lanes : Anti-PICOT antibody (ab226396) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 10 secondsLysates prepared using NETN lysis buffer.
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PICOT was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226396 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226396 at 0.4 µg/ml.
Lane 1: ab226396 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab226396 has been referenced in 1 publication.
- Kang HG et al. Genetic variants in histone modification regions are associated with the prognosis of lung adenocarcinoma. Sci Rep 11:21520 (2021). PubMed: 34728688