Overview

  • Product name
    Picro Sirius Red Stain Kit (Connective Tissue Stain)
  • Sample type
    Tissue sections
  • Assay time
    1h 00m
  • Product overview

    Picro-Sirius Red Stain Kit  (Connective Tissue Stain) ab150681 is intended for use in the histological visualization of collagen I and III fibers in addition to muscle in tissue sections.


    The picro sirius red staining may be viewed using standard light microscopy.


    Picro sirius red staining has traditionally sometimes been used with polarized light to more easily differentiate the collagen fibres from the background. Studies have used the differences between the appearance of different collagen fibres under polarized light to differentiate between different types of collagen fibres; it is unclear if the differences in appearance of the fibres is due to orientation, thickness and packing of the fibres rather than reflecting different types of collagen fibers.


    Picro sirius red staining protocol summary:
    - deparaffinize sections if necessary and hydrate in distilled water
    - cover sections in picro-sirius red solution and incubate for 60 min
    - wash slide with acetic acid solution
    - wash slide with absolute alcohol
    - dehydrate, clear and mount slide

  • Notes

    Staining Interpretation

    Light Microscopy 

    Collagen Red
    Muscle Fibers    Yellow
    Cytoplasm Yellow

    Polarized Light Microscopy*

    See note in overview section

    Type I (Thick fibers) Yellow-Orange Birefringence
    Type III (Thin fibers) Green Birefringence

    Control Tissue: Lung, Uterus, Muscle, Kidney.

Properties

Images

  • ab150681 Picro Sirius Red Stain Kit (Connective Tissue Stain) staining formalin-fixed-paraffin embedded human skeletal muscle.

  • ab150681 (Picro Sirius Red stain) staining collagen (red), muscle fibres (yellow), cytoplasm (yellow) and red blood cells (yellow) in formalin fixed paraffin embedded normal human colon.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • ab150681 staining mouse liver sections in control (saline group) showing normal lobular structure; in CCl4 treated showing extensive collagen deposition and bridging fibrosis; and in CCl4-AND treated mice which showed decreased collagen deposition.

Protocols

References

This product has been referenced in:
  • Kramer AS  et al. InForm software: a semi-automated research tool to identify presumptive human hepatic progenitor cells, and other histological features of pathological significance. Sci Rep 8:3418 (2018). Read more (PubMed: 29467378) »
  • Fang S  et al. circHECTD1 promotes the silica-induced pulmonary endothelial-mesenchymal transition via HECTD1. Cell Death Dis 9:396 (2018). Read more (PubMed: 29540674) »
See all 41 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Great and easy to use kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Great kit. Easy to use and fast.
I would recommend it.

Picture shows staining in mouse heart tissue.

Abcam user community

Verified customer

Submitted Aug 26 2016

Answer

The Pico-sirius red stain is itself prepared in picric acid therefore I can confirm, the PSR solution does have picric acid in it. Please check the recipe in below links
http://jhc.sagepub.com/content/early/2014/07/31/0022155414545787.full.pdf+html
http://dbts.ucsf.edu/chen/Protocol/PicroSirius%20Red%20staining.pdf

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Answer

Any permanent mounting medium should work. We have used Fisher's "Permount".

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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