Key features and details
- Rabbit polyclonal to PIDD1
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-PIDD1 antibody
See all PIDD1 primary antibodies
DescriptionRabbit polyclonal to PIDD1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse
Recombinant fragment (His-T7-tag) corresponding to Mouse PIDD1 aa 666-915. Expressed in E.coli. N-terminal tags.
EMFEGEKFFAAFERGIDVDADRPDCVDGRICFVFYSHLKNVKEVYITTAL DREAQDVRGQVSFYRGSLPVEVPAEAEAARQRKGTDALWMATLPIKLPRL RGAQGSGQGTDFSLMPLNLGDAETGFLTQSNLLSVASRLGPDWPAVALHL GMPYHKLQRIRHEFRDDLDGQVRHMLFSWAERQTGQPGAVGHLVQALEQS DRRDVAEEVRAILELGRHKYQDSIRRTGLAPEDSTLPGTSASQTPESAQA
Database link: Q9ERV7
- IHC-P: Mouse kidney tissue. WB: Mouse brain lysate; Recombinant mouse PIDD1 protein.
This product was previously labelled as LRDD
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231315 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 101 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
RelevanceThe death domain (DD) containing protein PIDD is a p53 target gene in an erythroleukemia cell line that undergoes G1 phase arrest and subsequent apoptosis after p53 expression. Independently, PIDD was also described as a DD-containing protein with unknown function. The N-terminal region of PIDD contains seven leucine-rich repeats (LRRs), a protein interaction motif found in various proteins with diverse functions, followed by two ZU-5 domains and a C-terminal DD. PIDD forms a complex with caspase-2 and the adaptor protein RAIDD. Increased PIDD expression results in spontaneous activation of caspase-2 and sensitization to apoptosis by genotoxic stimuli, via interaction with caspase-2 and CRADD/RAIDD. PIDD also promotes apoptosis downstream of p53 as component of the DNA damage/stress response pathway that connects p53/TP53 to apoptosis. PIDD has also been shown to interact with NEMO/IKBKG and RIP1 and enhance sumoylation and ubiquitination of NEMO/IKBKG, an important component for activation of the transcription factor NF-kappa-B.
Cellular localizationCytoplasm. Nucleus.
- DKFZp434D229 antibody
- Leucine rich repeats and death domain containing antibody
- Leucine-rich repeats and death domain containing antibody
Formalin-fixed, paraffin-embedded mouse kidney tissue stained for PIDD1 using ab231315 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-PIDD1 antibody (ab231315) at 2 µg/ml + Mouse brain lysate
Predicted band size: 101 kDa
Anti-PIDD1 antibody (ab231315) at 2 µg/ml + Recombinant mouse PIDD1 protein
Predicted band size: 101 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231315 has not yet been referenced specifically in any publications.